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Angiotensin-(1-7) regulates the levels of angiotensin II receptor subtype AT1 mRNA differentially in a strain-specific fashion
- Source :
- Regulatory peptides. 95(1-3)
- Publication Year :
- 2000
-
Abstract
- Ang-(1-7) is an effector peptide of the renin-angiotensin system with several distinct actions that are likely mediated by a specific receptor. Regulatory effects of angiotensin (Ang) peptides, Ang-(1-7) and Ang II, on Ang receptor subtype 1 (AT1) mRNA expression were investigated in vascular smooth muscle cells (VSMC) from four University of Akron (Akr) rat strains (WKY, SHR and two backcross consomic lines SHR/y and SHR/a), and in SHR and WKY cells from Charles River Laboratories (Crl). In WKY/Akr and SHR/Akr, Ang-(1-7) treatment increased the levels of AT1 mRNA. This effect was inhibited by the specific Ang-(1-7) antagonist, A-779, in WKY/Akr but not SHR/Akr. Ang II had no effect in Akr cells, but it down-regulated AT1 mRNA in WKY/Crl and SHR/Crl VSMC. Ang-(1-7) did not affect AT1 mRNA levels in Crl lines. In conclusion, Ang-(1-7) regulates the AT1 receptor either directly or indirectly in a strain-specific fashion. The Ang-(1-7) antagonist, A-779, blocks the actions of Ang-(1-7) only in VSMC from WKY/Akr rats, suggesting either that the binding sites for Ang-(1-7) have different properties in SHR/Akr and WKY/Akr cell lines, or that some of the effects of Ang-(1-7) are not receptor mediated. Further, we found differences between Akr cells and Crl cells that are consistent with their genetic heterogeneity.
- Subjects :
- Male
medicine.medical_specialty
Angiotensin receptor
Vascular smooth muscle
Transcription, Genetic
Physiology
Clinical Biochemistry
Aorta, Thoracic
Biology
Biochemistry
Rats, Inbred WKY
Receptor, Angiotensin, Type 2
Muscle, Smooth, Vascular
Receptor, Angiotensin, Type 1
Cellular and Molecular Neuroscience
Endocrinology
Species Specificity
Internal medicine
Rats, Inbred SHR
Gene expression
Renin–angiotensin system
medicine
Animals
RNA, Messenger
Receptor
Antihypertensive Agents
Crosses, Genetic
Angiotensin II receptor type 1
Receptors, Angiotensin
Angiotensin II
Peptide Fragments
Rats
Gene Expression Regulation
Cell culture
cardiovascular system
Angiotensin I
hormones, hormone substitutes, and hormone antagonists
circulatory and respiratory physiology
Subjects
Details
- ISSN :
- 01670115
- Volume :
- 95
- Issue :
- 1-3
- Database :
- OpenAIRE
- Journal :
- Regulatory peptides
- Accession number :
- edsair.doi.dedup.....8cfd835ff9a2250fb853fe6c74f4af3a