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Evaluation of effect of alcoholic extract of heartwood of Pterocarpus marsupium on in vitro antioxidant, anti-glycation, sorbitol accumulation and inhibition of aldose reductase activity
- Source :
- Journal of Traditional and Complementary Medicine, Vol 7, Iss 3, Pp 307-314 (2017), Journal of Traditional and Complementary Medicine
- Publication Year :
- 2017
- Publisher :
- Elsevier, 2017.
-
Abstract
- Rising popularity of phytomedicines in various diseased conditions have strengthened the significance of plant-research and evaluation of phytoextracts in clinical manifestations. Pterocarpus marsupium Roxb., a medicinal plant, known for its anti-oxidant and anti-diabetic activity is a rich source of phytochemicals with antihyperglycemic and antihyperlipidemic activities. However, its possible role in diabetic complications is not evaluated yet. The present study explores the possible role of alcoholic extract of heartwood of P. marsupium in the treatment of long-term diabetic complications. The alcoholic extract of P. marsupium was evaluated for advanced glycation-end-products formation, erythrocyte sorbitol accumulation and rat kidney aldose reductase enzyme inhibition at the concentration of 25–400 μg/ml using in-vitro bioassays. Also the phytoextract at the concentration of 10–320 μg/ml was evaluated for its antioxidant potential by in-vitro antioxidant assays which includes, determination of total phenol content; reducing power assay; nitric oxide scavenging activity; superoxide radical scavenging activity; total antioxidant capacity; total flavonoid content; DPPH scavenging activity; and hydrogen peroxide scavenging activity. The alcoholic extract of P. marsupium across varying concentrations showed inhibitory effect as evident by IC50 on advanced glycation-end-products formation (55.39 μg/ml), sorbitol accumulation (151.00 μg/ml) and rat kidney aldose reductase (195.88 μg/ml). The phytoextract also exhibited high phenolic and flavonoid contents with promising antioxidant potential against the antioxidant assays evaluated. The present investigation suggests that the phytoextract showed prominent antioxidant, antiglycation property and, inhibited accumulation of sorbitol and ALR enzyme, thus promising a beneficial role in reducing/delaying diabetic complications.<br />Graphical abstract
- Subjects :
- 0301 basic medicine
Antioxidant
medicine.medical_treatment
Flavonoid
lcsh:Medicine
medicine.disease_cause
Nitric oxide
Diabetic complications
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Sorbitol accumulation
medicine
IC50
Anti-glycation
chemistry.chemical_classification
Aldose reductase
biology
Traditional medicine
lcsh:R
Pterocarpus marsupium
biology.organism_classification
ALR inhibition
030104 developmental biology
Complementary and alternative medicine
chemistry
Biochemistry
Oxidative stress
030220 oncology & carcinogenesis
Sorbitol
Original Article
Subjects
Details
- Language :
- English
- ISSN :
- 22254110
- Volume :
- 7
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Journal of Traditional and Complementary Medicine
- Accession number :
- edsair.doi.dedup.....8c6f5185fe964c2259b7d51385dce57d