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An approach to rescue the fertility of stallions with a high level of hemospermia
- Source :
- Scopus, Repositório Institucional da UNESP, Universidade Estadual Paulista (UNESP), instacron:UNESP
- Publication Year :
- 2020
- Publisher :
- Wiley, 2020.
-
Abstract
- Made available in DSpace on 2020-12-12T02:47:11Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-09-01 A high amount of blood and not the mere presence of blood in equine semen impacts fertility. The aim of this study was to develop an approach to rescue the fertility of stallions with high hemospermia levels. Semen from 15 stallions was divided into four experimental groups: (a) Control—pure raw semen, (b) WB50—50% (v/v) whole blood added into semen, (c) E1—WB50 extended in a 1:1 (v/v) ratio with milk-based extender and (d) E2—WB50 extended in a 2:1 ratio with milk-based extender. Sperm kinetics, plasma membrane integrity (PMI), lipid peroxidation (PER) and intracellular superoxide (O2) production were immediately evaluated. Four cycles of 20 mares were randomly assigned to the experimental groups. Mares were bred with an insemination dose of 1 billion total sperm and pregnancy was diagnosed 14 days after ovulation. Sperm kinetics could not be evaluated in the WB50 samples. Total motility was lower (p .05); however, O2 production was higher (p .05) for CT and E1 samples. The control (90%) and E2 (90%) groups had superior (p
- Subjects :
- Male
endocrine system
media_common.quotation_subject
Semen
Fertility
Biology
Insemination
sperm
law.invention
Andrology
penis
Endocrinology
blood
Pregnancy
Superoxides
law
Animals
Horses
Ovulation
Insemination, Artificial
Sperm motility
equine
media_common
urogenital system
Cell Membrane
Extender
semen
Hemospermia
Spermatozoa
Sperm
Sperm Motility
Female
Horse Diseases
Animal Science and Zoology
Lipid Peroxidation
Biotechnology
Subjects
Details
- ISSN :
- 14390531 and 09366768
- Volume :
- 55
- Database :
- OpenAIRE
- Journal :
- Reproduction in Domestic Animals
- Accession number :
- edsair.doi.dedup.....8c531d5ace1f2a20f119821e82d1e0a7