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Regulation of the cell cycle by B-Myb
- Source :
- Blood cells, moleculesdiseases. 27(2)
- Publication Year :
- 2001
-
Abstract
- B-Myb is a cell-cycle-regulated member of the Myb transcription factor and, like c-Myb, has been implicated in regulation of hematopoietic cell proliferation and differentiation. In this study we have examined the mechanisms by which B-Myb regulates the cell cycle. We found that the ability of B-Myb both to promote Saos-2 cells into the S phase of the cell cycle and to overcome G1 arrest mediated by overexpression of the retinoblastoma-related p107 protein was correlated with the capacity of B-Myb to form an in vivo complex with p107, but was independent of its transactivation function. Further experiments using a B-Myb dominant-negative protein suggested that transcriptional activation of genes regulated through Myb DNA-binding sequences was required for cell proliferation. Our experiments suggest, therefore, that B-Myb influences cell cycle progression at two distinct levels: by inhibiting p107 and by inducing transcription of specific target genes.
- Subjects :
- animal structures
Cyclin A
Cell Cycle Proteins
Transfection
Tumor Cells, Cultured
E2F1
Humans
MYB
Molecular Biology
Transcription factor
biology
Cell growth
fungi
Cell Cycle
S-phase-promoting factor
Cell Biology
Hematology
Cell cycle
Cell biology
DNA-Binding Proteins
Gene Expression Regulation
biology.protein
Cancer research
Trans-Activators
Molecular Medicine
Restriction point
Subjects
Details
- ISSN :
- 10799796
- Volume :
- 27
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Blood cells, moleculesdiseases
- Accession number :
- edsair.doi.dedup.....8c0eb2708d4ced039d1a89d393c26ddc