Back to Search
Start Over
Pharmacological characterization of mechanisms involved in the vasorelaxation produced by rosuvastatin in aortic rings from rats with a cafeteria‐style diet
- Source :
- Clinical and Experimental Pharmacology & Physiology
- Publication Year :
- 2015
- Publisher :
- Wiley, 2015.
-
Abstract
- The present study aimed to investigate the possible influence of several inhibitors and blockers on the vascular effect produced by the acute in vitro application of rosuvastatin to phenylephrine-precontracted aortic rings from rats with a semi-solid, cafeteria-style (CAF) diet. It also aimed to examine the effects of rosuvastatin on the expression of endothelial nitric oxide synthase (eNOS), inducible nitric oxide synthase, constitutive cyclooxygenase, and inducible cyclooxygenase in aortic rings from rats with a CAF diet. From comparisons of the effect on phenylephrine-precontracted aortic rings extracted from rats with two different diets (a standard and a CAF diet), it was found that 10(-9) -10(-5) -mol/L rosuvastatin produced lower concentration-dependent vasorelaxation on rings from the CAF diet group. The vasorelaxant effect was unaffected by the vehicle, but it was significantly attenuated by 10(-5) -mol/L N(G) -nitro-l-arginine methyl ester, 10(-2) -mol/L tetraethylammonium, 10(-3) -mol/L 4-aminopyridine, 10(-7) -mol/L apamin plus 10(-7) -mol/L charybdotoxin, 10(-5) -mol/L indomethacin, or 10(-5) -mol/L cycloheximide. Moreover, in aortic rings from rats with a CAF diet, rosuvastatin enhanced the expression of eNOS, inducible nitric oxide synthase, constitutive cyclooxygenase, and inducible cyclooxygenase. The acute in vitro application of rosuvastatin to phenylephrine-precontracted aortic rings from rats with a CAF diet had a vasorelaxant effect. Overall, the present results suggest that the stimulation of eNOS, the opening of Ca(2+) -activated and voltage-activated K(+) channels, the stimulation of prostaglandin synthesis and enhanced protein levels of eNOS, inducible nitric oxide synthase, constitutive cyclooxygenase, and inducible cyclooxygenase are involved in this relaxant effect.
- Subjects :
- Male
obesity
medicine.medical_specialty
endothelium
Nitric Oxide Synthase Type III
Endothelium
Physiology
Vasodilator Agents
Aorta, Thoracic
Apamin
chemistry.chemical_compound
Organ Culture Techniques
Enos
Physiology (medical)
Internal medicine
medicine
Animals
Rosuvastatin
Rats, Wistar
Rosuvastatin Calcium
Pharmacology
Dose-Response Relationship, Drug
biology
Chemistry
Original Articles
biology.organism_classification
rate
Diet
Rats
Vasodilation
Nitric oxide synthase
aorta
Endocrinology
medicine.anatomical_structure
biology.protein
Endothelium, Vascular
Cyclooxygenase
rosuvastatin
medicine.drug
Subjects
Details
- ISSN :
- 14401681 and 03051870
- Volume :
- 42
- Database :
- OpenAIRE
- Journal :
- Clinical and Experimental Pharmacology and Physiology
- Accession number :
- edsair.doi.dedup.....8bfccc1989485bfb1fd8445ecc52c379