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The Identification of Mono‐, Di‐, Tri‐, and Tetragalactosyl‐diacylglycerols and their Natural Estolides in Oat Kernels
- Source :
- Lipids. 43:533-548
- Publication Year :
- 2008
- Publisher :
- Wiley, 2008.
-
Abstract
- Oat kernels were extracted with methanol, and glycolipid-enriched fractions were prepared using silica solid phase extraction. Using direct infusion electrospray ionization (ESI) tandem mass spectrometry (MS), high performance liquid chromatography (HPLC)-ESI-MS, and HPLC-atmospheric pressure chemical ionization (APCI)-MS, we confirmed previous reports that digalactosyldiacylglycerol (DGDG) was the most abundant glycolipid in oat kernels and confirmed a previous report of the presence of a DGDG mono-estolide in oat kernels. In the current study we also identified several additional natural galactolipid estolides: two new DGDG estolides (di- and tri-estolides), two trigalactosyldiacylglycerol (TriGDG) estolides (mono- and di-estolides), and one tetragalactosyldiacylglycerol (TetraGDG) estolide (mono-estolide). The levels of total galactolipid estolides in oat kernels were estimated to be about 29% of the total glycolipid fraction. To our knowledge, this report is the first evidence of natural di- and tri-estolides of polar lipids.
- Subjects :
- Spectrometry, Mass, Electrospray Ionization
Chemical ionization
Chromatography
Galactolipid
Avena
Chemistry
Electrospray ionization
Organic Chemistry
Atmospheric-pressure chemical ionization
Cell Biology
Mass spectrometry
Tandem mass spectrometry
Biochemistry
High-performance liquid chromatography
Article
Diglycerides
Atmospheric Pressure
Solid phase extraction
Chromatography, High Pressure Liquid
Subjects
Details
- ISSN :
- 15589307 and 00244201
- Volume :
- 43
- Database :
- OpenAIRE
- Journal :
- Lipids
- Accession number :
- edsair.doi.dedup.....8bf45322c5ddc51e294ceb82ee377912