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Lacto-ghrestatin, a novel bovine milk-derived peptide, suppresses ghrelin secretion
- Source :
- FEBS Letters. 591:2121-2130
- Publication Year :
- 2017
- Publisher :
- Wiley, 2017.
-
Abstract
- Ghrelin, an endogenous peptide isolated from the stomach, is known to stimulate food intake after peripheral administration. We found that the enzymatic digest of β-lactoglobulin decreases ghrelin secretion from the ghrelin-producing cell line MGN3-1. The peptides present in the digest were comprehensively analyzed using the nanoLC-OrbitrapMS. Among them, we identified that the nonapeptide LIVTQTMKG, corresponding to β-lactoglobulin(1-9), suppresses ghrelin secretion from MGN3-1 cells. We named LIVTQTMKG 'lacto-ghrestatin'. We found that lacto-ghrestatin decreases intracellular cAMP levels and mRNA expression levels of ghrelin production-related genes in MGN3-1 cells. Orally administered lacto-ghrestatin decreases plasma ghrelin levels and food intake in fasted mice. Lacto-ghrestatin is the first food-derived peptide to suppress ghrelin secretion in vitro and in vivo.
- Subjects :
- Male
0301 basic medicine
medicine.medical_specialty
Intracellular Space
Biophysics
Administration, Oral
Peptide
Endogeny
Lactoglobulins
Biology
Biochemistry
Cell Line
Eating
Mice
03 medical and health sciences
Structural Biology
In vivo
Internal medicine
Cyclic AMP
Genetics
medicine
Animals
Amino Acid Sequence
Molecular Biology
chemistry.chemical_classification
Colforsin
digestive, oral, and skin physiology
Fasting
Cell Biology
Ghrelin
Peptide Fragments
In vitro
Milk
030104 developmental biology
Endocrinology
chemistry
Cell culture
Cattle
Digestion
hormones, hormone substitutes, and hormone antagonists
Intracellular
Ghrelin secretion
Subjects
Details
- ISSN :
- 00145793
- Volume :
- 591
- Database :
- OpenAIRE
- Journal :
- FEBS Letters
- Accession number :
- edsair.doi.dedup.....8be8637ded16c127d12258ef4aa3b606