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TR-DB: an open-access database of compounds affecting the ethylene-induced triple response in Arabidopsis
- Source :
- Plant physiology and biochemistry : PPB. 75
- Publication Year :
- 2014
-
Abstract
- Small molecules which act as hormone agonists or antagonists represent useful tools in fundamental research and are widely applied in agriculture to control hormone effects. High-throughput screening of large chemical compound libraries has yielded new findings in plant biology, with possible future applications in agriculture and horticulture. To further understand ethylene biosynthesis/signaling and its crosstalk with other hormones, we screened a 12,000 compound chemical library based on an ethylene-related bioassay of dark-grown Arabidopsis thaliana (L.) Heynh. seedlings. From the initial screening, 1313 (∼11%) biologically active small molecules altering the phenotype triggered by the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC), were identified. Selection and sorting in classes were based on the angle of curvature of the apical hook, the length and width of the hypocotyl and the root. A MySQL-database was constructed (https://chaos.ugent.be/WE15/) including basic chemical information on the compounds, images illustrating the phenotypes, phenotype descriptions and classification. The research perspectives for different classes of hit compounds will be evaluated, and some general screening tips for customized high-throughput screening and pitfalls will be discussed.
- Subjects :
- Databases, Factual
Physiology
Arabidopsis
Amino Acids, Cyclic
Plant Science
Biology
Plant Roots
Chemical library
chemistry.chemical_compound
Plant Growth Regulators
Auxin
Gene Expression Regulation, Plant
Genetics
chemistry.chemical_classification
Drug discovery
Arabidopsis Proteins
Biological activity
Ethylenes
biology.organism_classification
Phenotype
Small molecule
Hypocotyl
Biochemistry
chemistry
Seedlings
Chemical genetics
Subjects
Details
- ISSN :
- 18732690
- Volume :
- 75
- Database :
- OpenAIRE
- Journal :
- Plant physiology and biochemistry : PPB
- Accession number :
- edsair.doi.dedup.....8bdeba01806cd44ff65b8bddef08e921