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Abscisic Acid Is Required for Root Elongation Associated With Ca2+ Influx in Response to Water Stress
- Source :
- Frontiers in Plant Science, Frontiers in Plant Science, Vol 11 (2020)
- Publication Year :
- 2020
- Publisher :
- Frontiers Media S.A., 2020.
-
Abstract
- Abscisic acid (ABA) is a critical hormone for plant survival under water stress. In this study, large-scale mutants of the Arabidopsis ecotype Columbia-0 (Col-0) were generated by ethyl methanesulfonate (EMS)-mutagenesis, and an improved root elongation under water-stress 1 (irew1) mutant showing significantly enhanced root growth was isolated under a water potential gradient assay. Then, irew1 and ABA-related mutants in Arabidopsis or tomato plants were observed under water potential gradient assay or water-deficient conditions. ABA pathway, Ca2+ response, and primary root (PR) elongation rate were monitored in addition to DNA- and RNA-Seq analyses. We found that based on phenotyping and transcriptional analyses, irew1 exhibited enhanced PR growth, ABA, and Ca2+ responses, compared to wild type subjected to water stress. Interestingly, exogenous Ca2+ application enhanced PR growth of irew1, ABA-biosynthesis deficient mutants in Arabidopsis, and tomato plants, in response to water potential gradients or water-deficient conditions. In combination with other ABA-related mutants and pharmacological studies, our results suggest that ABA is required for root elongation associated with Ca2+ influx in response to water stress.
- Subjects :
- Ethyl methanesulfonate
Arabidopsis thaliana
Physiology
Mutant
chemistry.chemical_element
Plant Science
Calcium
lcsh:Plant culture
tomato
abscisic acid
chemistry.chemical_compound
water stress
Arabidopsis
Genetics
lcsh:SB1-1110
Abscisic acid
Original Research
calcium
Ecotype
biology
fungi
Wild type
food and beverages
biology.organism_classification
root
Retraction
Cell biology
chemistry
Elongation
Subjects
Details
- Language :
- English
- ISSN :
- 1664462X
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Frontiers in Plant Science
- Accession number :
- edsair.doi.dedup.....8bdaa038ea932bb099376ba17e1b7bc2