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High genetic similarity between non-typhoidal Salmonella isolated from paired blood and stool samples of children in the Democratic Republic of the Congo
- Source :
- PLoS Neglected Tropical Diseases, PLoS Neglected Tropical Diseases, Vol 14, Iss 7, p e0008377 (2020)
- Publication Year :
- 2020
- Publisher :
- PUBLIC LIBRARY SCIENCE, 2020.
-
Abstract
- Background Non-typhoidal Salmonella (NTS) serotypes Typhimurium and Enteritidis are a major cause of bloodstream infections in children in sub-Saharan Africa but their reservoir is unknown. We compared pairs of NTS blood and stool isolates (with the same NTS serotype recovered in the same patient) for genetic similarity. Methods Between November 2013 and April 2017, hospital-admitted children (29 days to 14 years) with culture-confirmed NTS bloodstream infections were enrolled in a cross-sectional study at Kisantu Hospital, DR Congo. Stool cultures for Salmonella were performed on a subset of enrolled children, as well as on a control group of non-febrile hospital-admitted children. Pairs of blood and stool NTS isolates were assessed for genetic similarity by multiple-locus variable-number of tandem repeats (MLVA) and genomics analysis. Results A total of 299 children with NTS grown from blood cultures (Typhimurium 68.6%, Enteritidis 30.4%, other NTS 1.0%) had a stool sample processed; in 105 (35.1%) of them NTS was detected (Typhimurium 70.5%, Enteritidis 25.7%, other NTS 3.8%). A total of 87/105 (82.9%) pairs of blood and stool NTS isolates were observed (representing 29.1% of the 299 children). Among 1598 controls, the proportion of NTS stool excretion was 2.1% (p < 0.0001). MLVA types among paired isolates were identical in 82/87 (94.3%) pairs (27.4% of the 299 children; 61/66 (92.4%) in Typhimurium and 21/21 (100%) in Enteritidis pairs). Genomics analysis confirmed high genetic similarity within 41/43 (95.3%) pairs, showing a median SNP difference of 1 (range 0–77) and 1 (range 0–4) for Typhimurium and Enteritidis pairs respectively. Typhimurium and Enteritidis isolates belonged to sequence types ST313 lineage II and ST11 respectively. Conclusion Nearly 30% of children with NTS bloodstream infection showed stool excretion of an NTS isolate with high genetic similarity, adding to the evidence of humans as a potential reservoir for NTS.<br />Author summary Non-typhoidal Salmonella (NTS) is an important cause of bloodstream infections in sub-Saharan Africa, but little is known about its reservoir and transmission. We assessed the possibility of a human reservoir by culturing stool samples from children with NTS found in their blood. Next, we compared the obtained blood and stool NTS isolates for genetic similarity. We found that a high proportion of children with NTS in their blood also had NTS in their stool (35.1%) when compared to a control group of children without suspicion of bloodstream infection (2.1%). We observed that Salmonella Typhimurium and Salmonella Enteritidis were the main serotypes isolated from blood and stool, and that their proportions were similar within the different populations. We found that 94.3% of the paired NTS blood and stool isolates (or 27.4% of children with NTS in their blood and a stool culture done) were genetically similar to each other. This observation adds to the evidence of a potential human reservoir for NTS.
- Subjects :
- 0301 basic medicine
Serotype
Bacterial Diseases
Salmonella
Physiology
RC955-962
Non typhoidal salmonella
Bacteremia
Salmonella typhi
medicine.disease_cause
Pathology and Laboratory Medicine
Salmonella Typhi
DISEASE
Feces
0302 clinical medicine
Arctic medicine. Tropical medicine
Medicine and Health Sciences
NONTYPHOIDAL SALMONELLA
EPIDEMIOLOGY
Child
VERSION
TREE
Data Management
EXCRETION
SITES
Phylogenetic Analysis
Hematology
respiratory system
Body Fluids
Bacterial Pathogens
Phylogenetics
Blood
Infectious Diseases
Medical Microbiology
INFECTIONS
Child, Preschool
Salmonella Typhimurium
Public aspects of medicine
RA1-1270
Anatomy
Pathogens
Life Sciences & Biomedicine
Research Article
circulatory and respiratory physiology
AFRICA
Computer and Information Sciences
Adolescent
030231 tropical medicine
Excretion
Multiple Loci VNTR Analysis
Biology
Microbiology
Polymorphism, Single Nucleotide
03 medical and health sciences
Genetic similarity
Enterobacteriaceae
Tropical Medicine
medicine
Genetics
Humans
Evolutionary Systematics
Microbial Pathogens
Taxonomy
NON-TYPHOIDAL SALMONELLA
Evolutionary Biology
Science & Technology
Bacteria
Public Health, Environmental and Occupational Health
Organisms
Biology and Life Sciences
Infant
Bloodstream Infections
Human Genetics
Sequence types
030104 developmental biology
Cross-Sectional Studies
nervous system
Parasitology
Physiological Processes
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- PLoS Neglected Tropical Diseases, PLoS Neglected Tropical Diseases, Vol 14, Iss 7, p e0008377 (2020)
- Accession number :
- edsair.doi.dedup.....8b9da18d4de9ea4806ae1395b1df5bb8