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Protein kinase C autoimmunity in paraneoplastic cerebellar degeneration and non-small-cell lung cancer
- Source :
- Journal of Neurology, Neurosurgery & Psychiatry. 77:1359-1362
- Publication Year :
- 2006
- Publisher :
- BMJ, 2006.
-
Abstract
- Background: The clinical and immunological profiles of patients with paraneoplastic cerebellar degeneration (PCD) and non-small-cell lung cancer (NSCLC) are not well known. Objective: To review the clinical and immunological features of patients with PCD, NSCLC and without well-characterised onconeural antibodies. Methods: The clinical features of nine patients with the diagnosis of classical PCD and NSCLC, included in our archives, were retrospectively reviewed. The presence of antibodies to cerebellar components was determined by immunohistochemistry and immunoblot of rat cerebellum. A cDNA library of human cerebellum was screened with the positive sera to identify the antigen. Results: Nine patients with PCD and NSCLC were identified. Six patients were men, and the median age at diagnosis of PCD was 63 (range 47–73) years. PCD was completely reversed in two patients, and partially in one, after treatment of the tumour. The serum of one of the patients with PCD showed a unique reactivity with Purkinje cells. The screening of a cerebellar-expression library resulted in the isolation of protein kinase Cγ (PKCγ). PKCγ immunoreactivity was not observed in the serum of 170 patients with non-paraneoplastic neurological syndromes, 27 patients with PCD, no onconeural antibodies and small-cell lung cancer, and 52 patients with NSCLC without paraneoplastic neurological syndromes. The NSCLC from 11 patients without PCD did not express PKCγ at either the RNA or protein level. However, many cells of the NSCLC of the patient with PKCγ antibodies expressed PKCγ. Conclusion: PCD occurs in patients with NSCLC without typical onconeural antibodies and is associated with immune reactions against key proteins of the Purkinje cells.
- Subjects :
- Paper
Male
congenital, hereditary, and neonatal diseases and abnormalities
Cerebellum
Pathology
medicine.medical_specialty
Lung Neoplasms
medicine.disease_cause
Paraneoplastic Cerebellar Degeneration
Antibodies
Autoimmunity
Purkinje Cells
Antigen
Carcinoma, Non-Small-Cell Lung
otorhinolaryngologic diseases
medicine
Carcinoma
Humans
Lung cancer
Protein Kinase C
Aged
Retrospective Studies
biology
business.industry
Middle Aged
Paraneoplastic cerebellar degeneration
medicine.disease
Immunohistochemistry
respiratory tract diseases
Editorial Commentary
Psychiatry and Mental health
medicine.anatomical_structure
biology.protein
Female
Surgery
Neurology (clinical)
Antibody
business
Subjects
Details
- ISSN :
- 00223050
- Volume :
- 77
- Database :
- OpenAIRE
- Journal :
- Journal of Neurology, Neurosurgery & Psychiatry
- Accession number :
- edsair.doi.dedup.....8b9a5b1d207e09eaedb045f592b7974e