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Exploring the Remarkable Diversity of Culturable Escherichia coli Phages in the Danish Wastewater Environment
- Source :
- Viruses, Volume 12, Issue 9, Olsen, N S, Forero-Junco, L, Kot, W & Hansen, L H 2020, ' Exploring the Remarkable Diversity of Culturable Escherichia coli Phages in the Danish Wastewater Environment ', Viruses, vol. 12, no. 9, 986 . https://doi.org/10.3390/v12090986, Viruses, Vol 12, Iss 986, p 986 (2020)
- Publication Year :
- 2020
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2020.
-
Abstract
- Phages drive bacterial diversity, profoundly influencing microbial communities, from microbiomes to the drivers of global biogeochemical cycling. Aiming to broaden our understanding of Escherichia coli (MG1655, K-12) phages, we screened 188 Danish wastewater samples and isolated 136 phages. Ninety-two of these have genomic sequences with less than 95% similarity to known phages, while most map to existing genera several represent novel lineages. The isolated phages are highly diverse, estimated to represent roughly one-third of the true diversity of culturable virulent dsDNA Escherichia phages in Danish wastewater, yet almost half (40%) are not represented in metagenomic databases, emphasising the importance of isolating phages to uncover diversity. Seven viral families, Myoviridae, Siphoviridae, Podoviridae, Drexlerviridae, Chaseviridae, Autographviridae, and Microviridae, are represented in the dataset. Their genomes vary drastically in length from 5.3 kb to 170.8 kb, with a guanine and cytosine (GC) content ranging from 35.3% to 60.0%. Hence, even for a model host bacterium, substantial diversity remains to be uncovered. These results expand and underline the range of coliphage diversity and demonstrate how far we are from fully disclosing phage diversity and ecology.
- Subjects :
- 0301 basic medicine
BACTERIAL
RAREFACTION
DATABASE
Microviridae
VIRUSES
030106 microbiology
lcsh:QR1-502
BACTERIOPHAGES
Myoviridae
PHAGE
lcsh:Microbiology
diversity
Bacteriophage
Siphoviridae
03 medical and health sciences
Podoviridae
taxonomy
bacteriophage
Virology
Escherichia
Escherichia coli
genomics
COMPLETE GENOME SEQUENCE
Coliphage
wastewater
Genetics
coliphage
biology
EXTRAPOLATION
DNA
TAXONOMY
biology.organism_classification
030104 developmental biology
Infectious Diseases
Metagenomics
human activities
Subjects
Details
- Language :
- English
- ISSN :
- 19994915
- Database :
- OpenAIRE
- Journal :
- Viruses
- Accession number :
- edsair.doi.dedup.....8b6dc226e488b2fec1de66d3e747e02e
- Full Text :
- https://doi.org/10.3390/v12090986