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Circulating Cell-Free Tumor DNA Analysis of 50 Genes by Next-Generation Sequencing in the Prospective MOSCATO Trial
- Source :
- Clinical cancer research : an official journal of the American Association for Cancer Research. 22(12)
- Publication Year :
- 2015
-
Abstract
- Purpose: Liquid biopsies based on circulating cell-free DNA (cfDNA) analysis are described as surrogate samples for molecular analysis. We evaluated the concordance between tumor DNA (tDNA) and cfDNA analysis on a large cohort of patients with advanced or metastatic solid tumor, eligible for phase I trial and with good performance status, enrolled in MOSCATO 01 trial (clinical trial NCT01566019). Experimental Design: Blood samples were collected at inclusion and cfDNA was extracted from plasma for 334 patients. Hotspot mutations were screened using next-generation sequencing for 50 cancer genes. Results: Among the 283 patients with tDNA–cfDNA pairs, 121 had mutation in both, 99 in tumor only, 5 in cfDNA only, and for 58 patients no mutation was detected, leading to a 55.0% estimated sensitivity [95% confidence interval (CI), 48.4%–61.6%] at the patient level. Among the 220 patients with mutations in tDNA, the sensitivity of cfDNA analysis was significantly linked to the number of metastatic sites, albumin level, tumor type, and number of lines of treatment. A sensitivity prediction score could be derived from clinical parameters. Sensitivity is 83% in patients with a high score (≥8). In addition, we analyzed cfDNA for 51 patients without available tissue sample. Mutations were detected for 22 patients, including 19 oncogenic variants and 8 actionable mutations. Conclusions: Detection of somatic mutations in cfDNA is feasible for prescreening phase I candidates with a satisfactory specificity; overall sensitivity can be improved by a sensitivity score allowing to select patients for whom cfDNA constitutes a reliable noninvasive surrogate to screen mutations. Clin Cancer Res; 22(12); 2960–8. ©2016 AACR.
- Subjects :
- 0301 basic medicine
Oncology
Adult
Male
Cancer Research
medicine.medical_specialty
Adolescent
Concordance
DNA Mutational Analysis
Bioinformatics
medicine.disease_cause
DNA sequencing
Circulating Tumor DNA
03 medical and health sciences
Young Adult
0302 clinical medicine
Internal medicine
Neoplasms
medicine
Biomarkers, Tumor
Humans
Molecular Targeted Therapy
Prospective Studies
Prospective cohort study
Gene
Aged
Mutation
Performance status
Clinical Trials, Phase I as Topic
business.industry
Patient Selection
High-Throughput Nucleotide Sequencing
DNA, Neoplasm
Middle Aged
Clinical trial
030104 developmental biology
030220 oncology & carcinogenesis
Female
business
Subjects
Details
- ISSN :
- 15573265
- Volume :
- 22
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Clinical cancer research : an official journal of the American Association for Cancer Research
- Accession number :
- edsair.doi.dedup.....8b65a5cbd6884468b132235b930f2336