Sustained release of epigallocatechin-3-gallate from chitosan-based scaffolds to promote osteogenesis of mesenchymal stem cell

Epigallocatechin-3-gallate (EGCG) is a kind of flavonoids and has the ability to promote differentiation of mesenchymal stem cells (MSCs) into osteoblasts. However, the EGCG is easily metabolized by cells during cell culture, which reduces its bioavailability. Therefore, in this paper, EGCG-loaded chitosan nanoparticles (ECN) were fabricated and entrapped into chitosan/alginate (CS/Alg) scaffolds to form CS/Alg-ECN scaffolds for improving the bioavailability of EGCG. The human umbilical cord mesenchymal stem cells (HUMSCs) were cultured on CS/Alg-ECN scaffolds to induce osteogenic differentiation. The results indicated that the CS/Alg-ECN scaffolds continuously released EGCG for up to 16 days. Besides, these results suggested that CS/Alg-ECN scaffolds promoted osteoblast differentiation through activating Wnt/β-catenin signaling pathway. Collectively, this study demonstrated that the entrapment ECN into CS/Alg scaffolds was a promising strategy for promoting osteogenesis of MSCs.