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Immunohistochemical biomarker validation in highly selective needle biopsy microarrays derived from mpMRI-characterized prostates
- Source :
- The Prostate. 78:1229-1237
- Publication Year :
- 2018
- Publisher :
- Wiley, 2018.
-
Abstract
- Introduction Diagnosing prostate cancer routinely involves tissue biopsy and increasingly image guided biopsy using multiparametric MRI (mpMRI). Excess tissue after diagnosis can be used for research to improve the diagnostic pathway and the vertical assembly of prostate needle biopsy cores into tissue microarrays (TMAs) allows the parallel immunohistochemical (IHC) validation of cancer biomarkers in routine diagnostic specimens. However, tissue within a biopsy core is often heterogeneous and cancer is not uniformly present, resulting in needle biopsy TMAs that suffer from highly variable cancer detection rates that complicate parallel biomarker validation. Materials and methods The prostate cores with the highest tumor burden (in terms of Gleason score and/or maximum cancer core length) were obtained from 249 patients in the PICTURE trial who underwent transperineal template prostate mapping (TPM) biopsy at 5 mm intervals preceded by mpMRI. From each core, 2 mm segments containing tumor or benign tissue (as assessed on H&E pathology) were selected, excised and embedded vertically into a new TMA block. TMA sections were then IHC-stained for the routinely used prostate cancer biomarkers PSA, PSMA, AMACR, p63, and MSMB and assessed using the h-score method. H-scores in patient matched malignant and benign tissue were correlated with the Gleason grade of the original core and the MRI Likert score for the sampled prostate area. Results A total of 2240 TMA cores were stained and IHC h-scores were assigned to 1790. There was a statistically significant difference in h-scores between patient matched malignant and adjacent benign tissue that is independent of Likert score. There was no association between the h-scores and Gleason grade or Likert score within each of the benign or malignant groups. Conclusion The construction of highly selective TMAs from prostate needle biopsy cores is possible. IHC data obtained through this method are highly reliable and can be correlated with imaging. IHC expression patterns for PSA, PSMA, AMACR, p63, and MSMB are distinct in malignant and adjacent benign tissue but did not correlate with mpMRI Likert score.
- Subjects :
- Image-Guided Biopsy
Male
medicine.medical_specialty
Urology
030232 urology & nephrology
Endocrinology & Metabolism
03 medical and health sciences
Prostate cancer
0302 clinical medicine
Prostate
Biopsy
Biomarkers, Tumor
medicine
Humans
DIAGNOSTIC-ACCURACY
1112 Oncology and Carcinogenesis
MSMB
Oncology & Carcinogenesis
Science & Technology
tissue microarrays
Tissue microarray
medicine.diagnostic_test
business.industry
CONSTRUCTING TISSUE MICROARRAYS
Prostatic Neoplasms
Cancer
1103 Clinical Sciences
Urology & Nephrology
prostate cancer
medicine.disease
CANCER
Immunohistochemistry
Magnetic Resonance Imaging
SPECIMENS
PATHOLOGY
medicine.anatomical_structure
Oncology
030220 oncology & carcinogenesis
1114 Paediatrics and Reproductive Medicine
Cancer biomarkers
Radiology
Neoplasm Grading
business
Life Sciences & Biomedicine
MRI
Subjects
Details
- ISSN :
- 02704137
- Volume :
- 78
- Database :
- OpenAIRE
- Journal :
- The Prostate
- Accession number :
- edsair.doi.dedup.....8b4abbfe99f3c16845b8b317796b753f