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Molecular Techniques for Identifying HCC Origin and Biology After Orthotopic Liver Transplantation
- Source :
- Diagnostic Molecular Pathology. 15:90-94
- Publication Year :
- 2006
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2006.
-
Abstract
- Hepatocellular carcinoma (HCC) is the fifth most common cancer in the world. Liver transplantation represents the potentially curative treatment for small HCC. Recurrence after surgical resection and liver transplantation remains one of the major obstacles in further prolonging survival of patients with HCC. In the new liver, HCC might be of recipient or donor origin. One approach for investigating this question is by performing human identification and/or engraftment analysis. Distinction between recurrent and de novo HCC after orthotopic liver transplantation could allow for the development of important clinical and therapeutic strategies. Polymerase chain reaction amplification of highly polymorphic short tandem repeat DNA sequences, gene expression profiling, and fluorescence in situ hybridization were applied in a patient who developed a second HCC after orthotopic liver transplantation from an opposite gender donor. These techniques provided consistent evidence that the second HCC was a recurrence of the primary tumor.
- Subjects :
- Male
Carcinoma, Hepatocellular
Genotype
Orthotopic liver transplantation
medicine.medical_treatment
Liver transplantation
Biology
Polymerase Chain Reaction
Pathology and Forensic Medicine
law.invention
Dental Enamel Proteins
law
medicine
Humans
neoplasms
Molecular Biology
In Situ Hybridization, Fluorescence
Polymerase chain reaction
Oligonucleotide Array Sequence Analysis
Amelogenin
medicine.diagnostic_test
Gene Expression Profiling
Liver Neoplasms
Cancer
Neoplasms, Second Primary
DNA, Neoplasm
Cell Biology
Middle Aged
medicine.disease
Primary tumor
Tissue Donors
digestive system diseases
Liver Transplantation
Gene expression profiling
Tandem Repeat Sequences
Hepatocellular carcinoma
Cancer research
Female
Neoplasm Recurrence, Local
Fluorescence in situ hybridization
Subjects
Details
- ISSN :
- 10529551
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- Diagnostic Molecular Pathology
- Accession number :
- edsair.doi.dedup.....8b47d0773566cd50f2fa1310f13e9841