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Applying Harmonic Optical Microscopy for Spatial Alignment of Atrial Collagen Fibers
- Source :
- PLoS ONE, PLoS ONE, Vol 5, Iss 11, p e13917 (2010)
- Publication Year :
- 2010
- Publisher :
- Public Library of Science, 2010.
-
Abstract
- BACKGROUND: Atrial fibrosis creates a vulnerable tissue for atrial fibrillation (AF), but the spatial disarray of collagen fibers underlying atrial fibrosis is not fully elucidated. OBJECTIVE: This study hypothesizes that harmonics optical microscopy can illuminate the spatial mal-alignment of collagen fibers in AF via a layer-by-layer approach. PATIENTS AND METHODS: Atrial tissues taken from patients who underwent open-heart surgery were examined by harmonics optical microscopy. Using the two-dimensional Fourier transformation method, a spectral-energy description of image texture was constituted and its entropy was used to quantify the mal-alignment of collagen fibers. The amount of collagen fiber was derived from its area ratio to total atrial tissue in each image. Serum C-terminal pro-collagen pro-peptide (CICP), pro-matrix metalloproteinase-1 (pro-MMP-1), and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) were also evaluated. RESULTS: 46 patients were evaluated, including 20 with normal sinus rhythm and 26 with AF. The entropy of spectral-energy distribution of collagen alignment was significantly higher in AF than that in sinus rhythm (3.97 ± 0.33 vs. 2.80 ± 0.18, p
- Subjects :
- Male
Pathology
medicine.medical_specialty
Entropy
lcsh:Medicine
Pathology/Cellular Pathology
Collagen Type I
law.invention
Extracellular matrix
Imaging, Three-Dimensional
Optical microscope
Fibrosis
law
Cardiovascular Disorders/Arrhythmias, Electrophysiology, and Pacing
Microscopy
Atrial Fibrillation
medicine
Humans
cardiovascular diseases
Heart Atria
lcsh:Science
Aged
Fibrillation
Multidisciplinary
Microscopy, Confocal
Tissue Inhibitor of Metalloproteinase-1
Chemistry
lcsh:R
Atrial fibrillation
Cell Biology/Extra-Cellular Matrix
Middle Aged
medicine.disease
Peptide Fragments
Procollagen peptidase
Atrial fibrosis
cardiovascular system
lcsh:Q
Female
medicine.symptom
Matrix Metalloproteinase 1
Algorithms
Biomarkers
Procollagen
Biomedical engineering
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 5
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....8b3a52841b591250c2bf68edc401ac4d