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A rapid non‐target screening method for determining prohibited substances in human urine using liquid chromatography/high‐resolution tandem mass spectrometry
- Source :
- Drug Testing and Analysis. 11:382-391
- Publication Year :
- 2018
- Publisher :
- Wiley, 2018.
-
Abstract
- Target analysis using liquid chromatography-tandem mass spectrometry is applied for rapidly detecting various prohibited doping substances. Frequent modification is required as additional substances are prohibited. We developed and validated a non-target screening method requiring no further modification because it analyzes the full spectrum of data in fixed m/z ranges. Urine samples were extracted using solid-phase extraction and analyzed by employing a method that combines full scan and variable data independent acquisition using high-resolution mass spectrometry; and all prohibited substances in the urine samples were successfully detected using our screening method. The method was validated in terms of specificity (no interferences), recoveries (29%-131%), matrix effects (35%-237%), limites of detection (0.0002-100 ng/mL), and intra- and inter-day precisions (coefficients of variation lower than 25%). The applicability of this method to doping tests was evaluated by analyzing 14 urine samples. As a result, the non-target screening method is efficient for conducting anti-doping tests because it can be applied without any further modification to prohibited drugs as well as to unknown targets that can be prohibited in the future.
- Subjects :
- Pharmaceutical Science
High resolution
Urine
Mass spectrometry
Tandem mass spectrometry
Sensitivity and Specificity
01 natural sciences
Analytical Chemistry
03 medical and health sciences
0302 clinical medicine
Non target
Limit of Detection
Tandem Mass Spectrometry
Screening method
Humans
Environmental Chemistry
Data-independent acquisition
030216 legal & forensic medicine
Spectroscopy
Chromatography
Illicit Drugs
Chemistry
Solid Phase Extraction
010401 analytical chemistry
Extraction (chemistry)
0104 chemical sciences
Substance Abuse Detection
Chromatography, Liquid
Subjects
Details
- ISSN :
- 19427611 and 19427603
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Drug Testing and Analysis
- Accession number :
- edsair.doi.dedup.....8b34bed681f19fcf80fd0623db44f1c7