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Toxic and adjuvant effects of silica nanoparticles on ovalbumin-induced allergic airway inflammation in mice
- Source :
- Respiratory Research, RESPIRATORY RESEARCH(17)
- Publication Year :
- 2016
- Publisher :
- BioMed Central, 2016.
-
Abstract
- Background: Silica nanoparticles (SNPs) can easily enter in respiratory system via inhalation because of their low molecular weight and ease of dispersion. Toxicity and adverse effects of SNPs vary according to the physical characteristics of the particle. Methods: To evaluate the toxic and adjuvant effects of 3 types of SNPs in the airway system, six-week-old female BALB/c mice were intranasally administered 3 types of SNPs (spherical [S-SNP], mesoporous [M-SNP], and polyethylene glycol-conjugated [P-SNP]) alone or SNPs/ovalbumin (OVA), three times weekly for 2 weeks. Airway hyper-responsiveness (AHR), bronchoalveolar lavage fluid (BALF), cytokine levels, and histology of the lungs were analyzed. Results: The S-SNPs/OVA group and M-SNPs/OVA group showed significant AHR, compared to the control group. Among all SNP-treated groups, the group administered SNPs/OVA showed greater inflammatory cell infiltration in BALF, extensive pathological changes, and higher cytokine levels (IL-5, IL-13, IL-1 beta, and IFN-gamma) than those administered SNPs alone or saline/OVA. Conclusion: Exposure to SNPs alone and SNPs/OVA induced toxicity in the respiratory system. SNPs alone showed significant toxic effects on the airway system. Meanwhile, SNPs/OVA exerted adjuvant effects to OVA of inducing allergic airway inflammation. In particular, M-SNPs showed the most severe airway inflammation in both direct toxicity and adjuvant effect assays. P-SNPs induced less inflammation than the other types of SNPs in both models.
- Subjects :
- 0301 basic medicine
medicine.medical_treatment
Airway Inflammation
02 engineering and technology
Polyethylene Glycols
Nanoparticle
Respiratory system
Lung
Inhalation Exposure
Mice, Inbred BALB C
medicine.diagnostic_test
biology
Inhalation
PEGylation
Silica
respiratory system
021001 nanoscience & nanotechnology
Silicon Dioxide
Adjuvant effect
Toxicity
Cytokine
Cytokines
Female
medicine.symptom
Bronchial Hyperreactivity
Inflammation Mediators
0210 nano-technology
Adjuvant
Bronchoalveolar Lavage Fluid
Porosity
Pulmonary and Respiratory Medicine
Ovalbumin
Bronchoconstriction
Inflammation
03 medical and health sciences
medicine
Animals
business.industry
Research
Pneumonia
Disease Models, Animal
030104 developmental biology
Bronchoalveolar lavage
Immunology
biology.protein
Nanoparticles
business
Subjects
Details
- Language :
- English
- ISSN :
- 1465993X and 14659921
- Volume :
- 17
- Database :
- OpenAIRE
- Journal :
- Respiratory Research
- Accession number :
- edsair.doi.dedup.....8b26f47b2ef31b90fc878c8c36a354f1