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Co-regulation of CodY and (p)ppGpp synthetases on morphology and pathogenesis of Streptococcus suis
- Source :
- Microbiological research.
- Publication Year :
- 2018
-
Abstract
- CodY and (p)ppGpp synthetases are two important global regulators of bacteria. In some pathogens, such as Listeria monocytogenes, the GTP pool links these two regulatory systems, and introducing a codY mutant into the ΔrelA strain restored the pathogenicity of the attenuated ΔrelA mutant. In previous studies, we identified the (p)ppGpp synthetases (RelA and RelQ) and CodY of Streptococcus suis. To understand the interrelationships between these two regulators in S. suis, a ΔrelAΔrelQΔcodY mutant was constructed, and its growth, morphology, and pathogenicity were evaluated. Compared with ΔrelAΔrelQ, ΔcodY, its growth was very slow, but its chain length was partly restored to the wild-type length and its capsule became thick and rough. The adherence, invasion ability, and resistance to whole-blood killing in vitro of ΔrelAΔrelQΔcodY and its lethality and colonization ability in mice were clearly reduced, which differs from the effects of these mutations in L. monocytogenes. An analysis of gene expression showed that CodY interacted with the relA promoter in a GTP-independent manner to positively regulate the expression of relA. The introduction of a codY mutant into the ΔrelAΔrelQ strain further reduced the expression of virulence factors, which suggests a novel interaction between the (p)ppGpp synthetases and CodY. This study extends our understanding of the relationship between the (p)ppGpp-mediated stringent response and the regulation of CodY in S. suis.
- Subjects :
- GTP'
Streptococcus suis
Stringent response
Virulence Factors
Mutant
Virulence
medicine.disease_cause
Microbiology
Ligases
03 medical and health sciences
Mice
Listeria monocytogenes
Bacterial Proteins
Streptococcal Infections
Gene expression
medicine
Animals
Adhesins, Bacterial
Promoter Regions, Genetic
030304 developmental biology
0303 health sciences
biology
030306 microbiology
Gene Expression Regulation, Bacterial
biology.organism_classification
Disease Models, Animal
Mutation
Female
Guanosine Triphosphate
Transcriptome
Bacteria
Transcription Factors
Subjects
Details
- ISSN :
- 16180623
- Database :
- OpenAIRE
- Journal :
- Microbiological research
- Accession number :
- edsair.doi.dedup.....8b17f960328e98c1bfffae6bb1ccf607