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Gaussia Luciferase as a Reporter for Quorum Sensing in Staphylococcus aureus
- Source :
- Sensors, Volume 20, Issue 15, Sensors, Vol 20, Iss 4305, p 4305 (2020), Sensors (Basel, Switzerland)
- Publication Year :
- 2020
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2020.
-
Abstract
- Gaussia luciferase (GLuc) is a secreted protein with significant potential for use as a reporter of gene expression in bacterial pathogenicity studies. To date there are relatively few examples of its use in bacteriology. In this study we show that GLuc can be functionally expressed in the human pathogen Staphylococcus aureus and furthermore show that it can be used as a biosensor for the agr quorum sensing (QS) system which employs autoinducing peptides to control virulence. GLuc was linked to the P3 promoter of the S. aureusagr operon. Biosensor strains were validated by evaluation of chemical agent-mediated activation and inhibition of agr. Use of GLuc enabled quantitative assessment of agr activity. This demonstrates the utility of Gaussia luciferase for in vitro monitoring of agr activation and inhibition.
- Subjects :
- Staphylococcus aureus
agr
Operon
Gaussia luciferase
Virulence
Human pathogen
Biology
lcsh:Chemical technology
medicine.disease_cause
Biochemistry
Article
Analytical Chemistry
Microbiology
03 medical and health sciences
Gaussia
Bacterial Proteins
medicine
Bacteriology
Humans
lcsh:TP1-1185
Luciferase
Electrical and Electronic Engineering
Luciferases
Instrumentation
030304 developmental biology
0303 health sciences
030306 microbiology
quorum sensing
biochemical phenomena, metabolism, and nutrition
bacterial infections and mycoses
biology.organism_classification
Atomic and Molecular Physics, and Optics
Quorum sensing
Trans-Activators
bacteria
Subjects
Details
- Language :
- English
- ISSN :
- 14248220
- Database :
- OpenAIRE
- Journal :
- Sensors
- Accession number :
- edsair.doi.dedup.....8af83a464a18e43a798de7d4e2496742
- Full Text :
- https://doi.org/10.3390/s20154305