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The maternal serum metabolome by multisegment injection-capillary electrophoresis-mass spectrometry: a high-throughput platform and standardized data workflow for large-scale epidemiological studies
- Source :
- Nature Protocols. 16:1966-1994
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- A standardized data workflow is described for large-scale serum metabolomic studies using multisegment injection-capillary electrophoresis-mass spectrometry. Multiplexed separations increase throughput ( 75%) from a multi-ethnic cohort of pregnant women (n = 1,004). We outline a validated protocol implemented in four batches over a 7-month period that includes details on preventive maintenance, sample workup, data preprocessing and metabolite authentication. We achieve stringent quality control (QC) and robust batch correction of long-term signal drift with good mutual agreement for a wide range of metabolites, including serum glucose as compared to a clinical chemistry analyzer (mean bias = 11%, n = 668). Control charts for a recovery standard (mean CV = 12%, n = 2,412) and serum metabolites in QC samples (median CV = 13%, n = 202) demonstrate acceptable intermediate precision with a median intraclass coefficient of 0.87. We also report reference intervals for 53 serum metabolites from a diverse population of women in their second trimester of pregnancy. A standardized protocol and data workflow for high-throughput analysis of the maternal serum metabolome is outlined. It uses multisegment injection–capillary electrophoresis–mass spectrometry and is applied to a multi-ethnic cohort of pregnant women.
- Subjects :
- Blood Glucose
Quality Control
Serum
Canada
Metabolite
Capillary electrophoresis–mass spectrometry
Mass Spectrometry
General Biochemistry, Genetics and Molecular Biology
Cohort Studies
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Metabolomics
Pregnancy
Reference Values
Metabolome
Humans
Throughput (business)
030304 developmental biology
Ions
Protocol (science)
Principal Component Analysis
0303 health sciences
Chromatography
Electrophoresis, Capillary
Fasting
Reference Standards
High-Throughput Screening Assays
3. Good health
Epidemiologic Studies
Workflow
chemistry
Calibration
Cohort
Female
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 17502799 and 17542189
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- Nature Protocols
- Accession number :
- edsair.doi.dedup.....8af0ac09ac79dc5dbf6c725fd5e2e653