Prospective uses of recombinant Lactococcus lactis expressing both listeriolysin O and mutated internalin A from Listeria monocytogenes as a tool for DNA vaccination

In this study, Lactococcus lactis was engineered to express mutated internalin A on its surface and to secrete large amounts of listeriolysin O (LLO) in order to improve its potential as a vehicle for DNA vaccination. Western blotting experiments demonstrated that the bacterium expressed LLO in both the cytoplasmic and extracellular compartments, with higher quantities found in the culture supernatants. A hemolytic assay showed that the recombinant strain secreted 250 ng active LLO/mg total protein. This mInlA/LLO-producing strain of L. lactis may be used as an alternative tool in DNA vaccination against a number of infectious diseases or in cancer therapy. Fil: de Azevedo, M. S. P.. Universidade Federal de Minas Gerais; Brasil Fil: Santos Rocha, C.. Universidade Federal de Minas Gerais; Brasil Fil: Pereira, V. B.. Universidade Federal de Minas Gerais; Brasil Fil: de Oliveira Junior, A. .. Universidade Federal de Minas Gerais; Brasil Fil: de Sousa, C. S.. Universidade Federal de Minas Gerais; Brasil Fil: Azevedo, V.. Universidade Federal de Minas Gerais; Brasil Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Chatel, J. M.. Institut National de la Recherche Agronomique; Francia Fil: Miyoshi, A.. Universidade Federal de Minas Gerais; Brasil