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IL-3 Induces Basophil Expansion In Vivo by Directing Granulocyte-Monocyte Progenitors to Differentiate into Basophil Lineage-Restricted Progenitors in the Bone Marrow and by Increasing the Number of Basophil/Mast Cell Progenitors in the Spleen
- Source :
- The Journal of Immunology. 182:2835-2841
- Publication Year :
- 2009
- Publisher :
- The American Association of Immunologists, 2009.
-
Abstract
- Recent work has established important roles for basophils in regulating immune responses. To exert their biological functions, basophils need to be expanded to critical numbers. However, the mechanisms underlying basophil expansion remain unclear. In this study, we established that IL-3 played an important role in the rapid and specific expansion of basophils. We found that the IL-3 complex (IL-3 plus anti-IL-3 Ab) greatly facilitated the differentiation of GMPs into basophil lineage-restricted progenitors (BaPs) but not into eosinophil lineage-restricted progenitors or mast cells in the bone marrow. We also found that the IL-3 complex treatment resulted in ∼4-fold increase in the number of basophil/mast cell progenitors (BMCPs) in the spleen. IL-3-driven basophil expansion depended on STAT5 signaling. We showed that GMPs but not common myeloid progenitors expressed low levels of IL-3 receptor. IL-3 receptor expression was dramatically up-regulated in BaPs but not eosinophil lineage-restricted progenitors. Approximately 38% of BMCPs expressed the IL-3Rα-chain. The up-regulated IL-3 receptor expression was not affected by IL-3 or STAT5. Our findings demonstrate that IL-3 induced specific expansion of basophils by directing GMPs to differentiate into BaPs in the bone marrow and by increasing the number of BMCPs in the spleen.
- Subjects :
- Receptor expression
Immunology
Mice, Transgenic
chemical and pharmacologic phenomena
Biology
Basophil
Granulocyte-Macrophage Progenitor Cells
Article
Leukocyte Count
Mice
medicine
Animals
Immunology and Allergy
Cell Lineage
Granulocyte Precursor Cells
Mast Cells
Progenitor cell
Myeloid Progenitor Cells
Mice, Knockout
Monocyte
Cell Differentiation
hemic and immune systems
Eosinophil
Mast cell
Receptors, Interleukin-3
Basophils
Up-Regulation
Mice, Inbred C57BL
medicine.anatomical_structure
Gene Expression Regulation
Interleukin-3
Bone marrow
Spleen
Subjects
Details
- ISSN :
- 15506606 and 00221767
- Volume :
- 182
- Database :
- OpenAIRE
- Journal :
- The Journal of Immunology
- Accession number :
- edsair.doi.dedup.....8ab6d95bdbcdc082e823e2741ad9d8fc