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Evidence of reduced single-stranded testicular sperm DNA from obstructive azoospermic men after 3 days of in-vitro culture
- Source :
- Human Reproduction. 16:1200-1203
- Publication Year :
- 2001
- Publisher :
- Oxford University Press (OUP), 2001.
-
Abstract
- The aim of the present study was to verify whether culturing testicular tissue, to obtain a higher percentage of motile spermatozoa and a better post-thaw recovery rate, affected the ratio between single/double-stranded sperm DNA and, consequently, DNA sensitivity to damage. Testicular biopsy samples from men with obstructive and secretory azoospermia, candidates for assisted reproductive treatment, were cultured for 72 h. The percentage of motile spermatozoa and the single/double stranded DNA ratio were assessed on the day of retrieval (day 0) and again on day 3. The single/double stranded DNA ratio was measured by the acridine orange (AO) staining method. Spermatozoa were classified as green (double-stranded chromatin) or red fluorescing (single-stranded chromatin). In obstructive azoospermia, median motility was 22% (range 10-44%) on day 0 and 50% (range 38-63%) on day 3 (P < 0.01). The median percentage of red stained spermatozoa was 53.5% (range 0.1-88%) on day 0 and 20% (range 2.7-99.9%) on day 3 (P < 0.05). No changes were observed in secretory azoospermia. The culture procedure from obstructive azoospermia not only increased the post-thaw recovery rate, as previously observed, but also reduced the portion of spermatozoa containing single-stranded DNA, thereby increasing the availability of double-stranded DNA spermatozoa for ICSI use.
- Subjects :
- Male
endocrine system
Time Factors
DNA damage
Biopsy
DNA, Single-Stranded
Obstructive azoospermia
Testicle
Biology
Andrology
chemistry.chemical_compound
Testis
medicine
Humans
Cells, Cultured
reproductive and urinary physiology
Sperm motility
Azoospermia
Sperm Count
Staining and Labeling
urogenital system
Rehabilitation
Acridine orange
Obstetrics and Gynecology
Oligospermia
medicine.disease
Spermatozoa
Acridine Orange
Staining
Chromatin
medicine.anatomical_structure
Reproductive Medicine
chemistry
Immunology
Sperm Motility
DNA Damage
Subjects
Details
- ISSN :
- 14602350
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- Human Reproduction
- Accession number :
- edsair.doi.dedup.....8aa9199194fec139e979352052932e5e