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Novel protocol for acute in situ ecotoxicity test using native crustaceans applied to groundwater ecosystems

Authors :
Florian Malard
Ana Sofia P. S. Reboleira
Andrea Castaño-Sánchez
Gabriela Kalčíková
Repositório da Universidade de Lisboa
Source :
Water, vol. 13, no. 8, 1132, 2021., Repositório Científico de Acesso Aberto de Portugal, Repositório Científico de Acesso Aberto de Portugal (RCAAP), instacron:RCAAP, Water, Vol 13, Iss 1132, p 1132 (2021), Water, Volume 13, Issue 8, Castaño-sánchez, A, Malard, F, Kalčíková, G & Reboleira, A S P S 2021, ' Novel Protocol for Acute In Situ Ecotoxicity Test Using Native Crustaceans Applied to Groundwater Ecosystems ', Water, vol. 13, no. 8, 1132 . https://doi.org/10.3390/w13081132
Publication Year :
2022
Publisher :
MDPI, 2022.

Abstract

Current standardized laboratory test protocols use model species that have limitations to accurately assess native species responses to stressors. We developed and tested a novel acute insitu protocol for testing field-collected organisms. We used Asellus aquaticus and NaCl as a reference toxicant to test for the effects of location (laboratory vs. insitu), medium (synthetic vs. field water), substrate (presence vs. absence), and protocol replicability. We further tested the protocol using groundwater-adapted isopods: Proasellus assaforensis for the effect of location, P. cavaticus of medium and P.lusitanicus of substrate. Our results showed that A.aquaticus’ lethality obtained with the novel acute insitu protocol did not significantly differ from those from laboratory testing. However, laboratory tested P.assaforensis showed a higher sensitivity, suggesting that its acclimation to laboratory conditions might have pernicious effects. A. aquaticus and P. cavaticus showed a higher mortality using synthetic medium insitu and under laboratory conditions, which overestimated the stressor’s effect. Besides, substrate use had no significant effect. The novel acute insitu protocol allows the use of native species under realistic scenarios. It is particularly well adapted for assessing the risk of groundwater ecosystems but it can be applied to a wide range of ecosystems.

Details

Language :
English
ISSN :
20734441
Database :
OpenAIRE
Journal :
Water, vol. 13, no. 8, 1132, 2021., Repositório Científico de Acesso Aberto de Portugal, Repositório Científico de Acesso Aberto de Portugal (RCAAP), instacron:RCAAP, Water, Vol 13, Iss 1132, p 1132 (2021), Water, Volume 13, Issue 8, Castaño-sánchez, A, Malard, F, Kalčíková, G & Reboleira, A S P S 2021, ' Novel Protocol for Acute In Situ Ecotoxicity Test Using Native Crustaceans Applied to Groundwater Ecosystems ', Water, vol. 13, no. 8, 1132 . https://doi.org/10.3390/w13081132
Accession number :
edsair.doi.dedup.....8aa0e6a2874f1c7bb7df6a962fcc1d0e