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Novel protocol for acute in situ ecotoxicity test using native crustaceans applied to groundwater ecosystems
- Source :
- Water, vol. 13, no. 8, 1132, 2021., Repositório Científico de Acesso Aberto de Portugal, Repositório Científico de Acesso Aberto de Portugal (RCAAP), instacron:RCAAP, Water, Vol 13, Iss 1132, p 1132 (2021), Water, Volume 13, Issue 8, Castaño-sánchez, A, Malard, F, Kalčíková, G & Reboleira, A S P S 2021, ' Novel Protocol for Acute In Situ Ecotoxicity Test Using Native Crustaceans Applied to Groundwater Ecosystems ', Water, vol. 13, no. 8, 1132 . https://doi.org/10.3390/w13081132
- Publication Year :
- 2022
- Publisher :
- MDPI, 2022.
-
Abstract
- Current standardized laboratory test protocols use model species that have limitations to accurately assess native species responses to stressors. We developed and tested a novel acute insitu protocol for testing field-collected organisms. We used Asellus aquaticus and NaCl as a reference toxicant to test for the effects of location (laboratory vs. insitu), medium (synthetic vs. field water), substrate (presence vs. absence), and protocol replicability. We further tested the protocol using groundwater-adapted isopods: Proasellus assaforensis for the effect of location, P. cavaticus of medium and P.lusitanicus of substrate. Our results showed that A.aquaticus’ lethality obtained with the novel acute insitu protocol did not significantly differ from those from laboratory testing. However, laboratory tested P.assaforensis showed a higher sensitivity, suggesting that its acclimation to laboratory conditions might have pernicious effects. A. aquaticus and P. cavaticus showed a higher mortality using synthetic medium insitu and under laboratory conditions, which overestimated the stressor’s effect. Besides, substrate use had no significant effect. The novel acute insitu protocol allows the use of native species under realistic scenarios. It is particularly well adapted for assessing the risk of groundwater ecosystems but it can be applied to a wide range of ecosystems.
- Subjects :
- 0106 biological sciences
In situ
ekologija
in situ exposure
Geography, Planning and Development
site-specific assessment
groundwater ecology
010501 environmental sciences
Aquatic Science
010603 evolutionary biology
01 natural sciences
Biochemistry
Acclimatization
chemistry.chemical_compound
ekotoksikologija
udc:628.112:502/504
Asellus aquaticus
Ecosystem
groundwater ecotoxicology
TD201-500
0105 earth and related environmental sciences
Water Science and Technology
podtalnica
Water supply for domestic and industrial purposes
biology
Hydraulic engineering
Proasellus
biology.organism_classification
Substrate (marine biology)
chemistry
Environmental chemistry
stygofauna
Environmental science
Ecotoxicity
TC1-978
Toxicant
Subjects
Details
- Language :
- English
- ISSN :
- 20734441
- Database :
- OpenAIRE
- Journal :
- Water, vol. 13, no. 8, 1132, 2021., Repositório Científico de Acesso Aberto de Portugal, Repositório Científico de Acesso Aberto de Portugal (RCAAP), instacron:RCAAP, Water, Vol 13, Iss 1132, p 1132 (2021), Water, Volume 13, Issue 8, Castaño-sánchez, A, Malard, F, Kalčíková, G & Reboleira, A S P S 2021, ' Novel Protocol for Acute In Situ Ecotoxicity Test Using Native Crustaceans Applied to Groundwater Ecosystems ', Water, vol. 13, no. 8, 1132 . https://doi.org/10.3390/w13081132
- Accession number :
- edsair.doi.dedup.....8aa0e6a2874f1c7bb7df6a962fcc1d0e