Back to Search
Start Over
Association of β1 Integrin with Focal Adhesion Kinase and Paxillin in Differentiating Schwann Cells
- Source :
- The Journal of Neuroscience. 20:3776-3784
- Publication Year :
- 2000
- Publisher :
- Society for Neuroscience, 2000.
-
Abstract
- Schwann cells (SCs) differentiate into a myelinating cell when simultaneously adhering to an axon destined for myelination and basal lamina. We are interested in defining the signaling pathway activated by basal lamina. Using SC/sensory neuron (N) cocultures, we identified β1 integrin and F-actin as components of a pathway leading to myelin gene expression and myelination (Fernandez-Valle et al., 1994, 1997). Here, we show that focal adhesion kinase (FAK) and paxillin are constitutively expressed by SCs contacting axons in the absence of basal lamina. Tyrosine phosphorylation of FAK and paxillin increases as SCs form basal lamina and differentiate. FAK and paxillin specifically coimmunoprecipitate with β1 integrin in differentiating SC/N cocultures but not SC-only cultures. Paxillin coimmunoprecipitates with FAK and fyn kinase in differentiating SC/N cocultures. A subset of tyrosine-phosphorylated β1 integrin, FAK, and paxillin molecules reside in the insoluble, F-actin-rich fraction of differentiating cocultures. Cytochalasin D, an actin depolymerizing agent, decreases tyrosine phosphorylation of FAK and paxillin and their association with β1 integrin and causes a dose-dependent increase in the abundance of insoluble FAK and paxillin complexes. Collectively, our work indicates that β1 integrin, FAK, paxillin, and fyn kinase form an actin-associated complex in SCs adhering to basal lamina in the presence of axons. This complex may be important for initiating the process of SC differentiation into a myelinating cell.
- Subjects :
- Cytochalasin D
PTK2
Cell Communication
macromolecular substances
environment and public health
Antibodies
Rats, Sprague-Dawley
Focal adhesion
chemistry.chemical_compound
FYN
medicine
Animals
Neurons, Afferent
ARTICLE
Phosphorylation
Cytoskeleton
Cells, Cultured
Paxillin
Nucleic Acid Synthesis Inhibitors
biology
Integrin beta1
General Neuroscience
Cell Differentiation
Tyrosine phosphorylation
Protein-Tyrosine Kinases
Phosphoproteins
Precipitin Tests
Axons
Rats
Cell biology
Cytoskeletal Proteins
enzymes and coenzymes (carbohydrates)
medicine.anatomical_structure
Animals, Newborn
nervous system
chemistry
Focal Adhesion Kinase 1
Focal Adhesion Protein-Tyrosine Kinases
biology.protein
Tyrosine
Basal lamina
Schwann Cells
biological phenomena, cell phenomena, and immunity
Signal transduction
Protein Binding
Signal Transduction
Subjects
Details
- ISSN :
- 15292401 and 02706474
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- The Journal of Neuroscience
- Accession number :
- edsair.doi.dedup.....8a73abf4d51169851e268598ae8978c7
- Full Text :
- https://doi.org/10.1523/jneurosci.20-10-03776.2000