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Biodegradable thermosensitive injectable PEG-PCL-PEG hydrogel for bFGF antigen delivery to improve humoral immunity
- Source :
- Growth factors (Chur, Switzerland). 27(6)
- Publication Year :
- 2009
-
Abstract
- In this contribution, a biodegradable and injectable thermosensitive poly(ethylene glycol)-poly(epsilon-caprolactone)-poly(ethylene glycol) (PEG-PCL-PEG, PECE) hydrogel system was successfully prepared for basic fibroblastic growth factor (bFGF) antigen delivery. bFGF encapsulated PECE hydrogel system (bFGF-hydrogel) is an injectable free-flowing sol at ambient temperature, and forms a non-flowing gel at physiological temperature acting as antigen depot. Furthermore, the cytotoxicity results showed that the PECE hydrogel could be regarded as a safe carrier, and bFGF could be released from the hydrogel system in an extended period in vitro. Otherwise, the immunogenicity of bFGF was improved significantly after encapsulated into the hydrogel. Strong humoral immunity created by bFGF-hydrogel was maintained for more than 14 weeks. Therefore, the prepared bFGF loaded PECE hydrogel might have great potential as a novel vaccine adjuvant for protein antigen.
- Subjects :
- Hot Temperature
Polyesters
Clinical Biochemistry
Biocompatible Materials
macromolecular substances
Pharmacology
complex mixtures
Antibodies
Hydrogel, Polyethylene Glycol Dimethacrylate
Injections
Polyethylene Glycols
chemistry.chemical_compound
Mice
Endocrinology
Drug Delivery Systems
L Cells
Antigen
Animals
Humans
Antigens
Cytotoxicity
Mice, Inbred BALB C
Vaccines
Immunogenicity
technology, industry, and agriculture
Cell Biology
Molecular biology
Controlled release
In vitro
Immunity, Humoral
PECE
chemistry
Delayed-Action Preparations
Humoral immunity
cardiovascular system
Female
Fibroblast Growth Factor 2
Ethylene glycol
Subjects
Details
- ISSN :
- 10292292
- Volume :
- 27
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Growth factors (Chur, Switzerland)
- Accession number :
- edsair.doi.dedup.....8a647e38eedcafc7ed002714beb42772