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IRF5 gene polymorphisms in melanoma
- Source :
- Journal of Translational Medicine, Journal of Translational Medicine, Vol 10, Iss 1, p 170 (2012)
- Publication Year :
- 2012
- Publisher :
- BioMed Central, 2012.
-
Abstract
- Background Interferon regulatory factor (IRF)-5 is a transcription factor involved in type I interferon signaling whose germ line variants have been associated with autoimmune pathogenesis. Since relationships have been observed between development of autoimmunity and responsiveness of melanoma to several types of immunotherapy, we tested whether polymorphisms of IRF5 are associated with responsiveness of melanoma to adoptive therapy with tumor infiltrating lymphocytes (TILs). Methods 140 TILs were genotyped for four single nucleotide polymorphisms (rs10954213, rs11770589, rs6953165, rs2004640) and one insertion-deletion in the IRF5 gene by sequencing. Gene-expression profile of the TILs, 112 parental melanoma metastases (MM) and 9 cell lines derived from some metastases were assessed by Affymetrix Human Gene ST 1.0 array. Results Lack of A allele in rs10954213 (G > A) was associated with non-response (p in vitro between cell lines carrying or not the A allele could be applied to the transcriptional profile of 112 melanoma metastases to predict their responsiveness to therapy, suggesting that IRF5 genotype may influence immune responsiveness by affecting the intrinsic biology of melanoma. Conclusions This study is the first to analyze associations between melanoma immune responsiveness and IRF5 polymorphism. The results support a common genetic basis which may underline the development of autoimmunity and melanoma immune responsiveness.
- Subjects :
- medicine.medical_treatment
lcsh:Medicine
Single-nucleotide polymorphism
Biology
Polymerase Chain Reaction
Polymorphism, Single Nucleotide
General Biochemistry, Genetics and Molecular Biology
medicine
Humans
Allele
Neoplasm Metastasis
Melanoma
DNA Primers
Medicine(all)
Base Sequence
Tumor-infiltrating lymphocytes
Biochemistry, Genetics and Molecular Biology(all)
Research
Gene Expression Profiling
lcsh:R
General Medicine
Immunotherapy
medicine.disease
Gene expression profiling
Immunology
Interferon Regulatory Factors
Cancer research
IRF5
Interferon regulatory factors
Subjects
Details
- Language :
- English
- ISSN :
- 14795876
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Journal of Translational Medicine
- Accession number :
- edsair.doi.dedup.....8a551e9701331ed4cb5a429ebfbc4f66