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The specific production of the third component of complement by osteoblastic cells treated with 1 alpha,25-dihydroxyvitamin D3
- Source :
- FEBS letters. 285(1)
- Publication Year :
- 1991
-
Abstract
- A 190 kDa protein was purified from conditioned media of mouse marrow-derived stromal cell (ST2) cultures treated with 1 alpha,25-dihydroxyvitamin D3 (1 alpha,25(OH)2D3) and identified as the third component of mouse complement (C3). Northern and Western blot analysis revealed that the production of C3 by ST2 and primary osteoblastic cells was strictly dependent on 1 alpha,25(OH)2D3, but the production by hepatocytes was not. Adding 1 alpha,25(OH)2D3 together with mouse C3 antibody to bone marrow cultures greatly inhibited the formation of tartrate-resistant acid phosphatase (TRAP)-positive osteoclast-like multinucleated cells. Adding C3 alone induced no TRAP-positive cell formation. These results suggest that, in bone tissues, C3 is specifically produced by osteoblasts in response to 1 alpha,25(OH)2D3 and somehow involved in inducing differentiation of bone marrow cells into osteoclasts in concert with other factors produced by osteoblasts in response to 1 alpha,25(OH)2D3.
- Subjects :
- medicine.medical_specialty
Stromal cell
1α,25-Dihydroxyvitamin D3
Cellular differentiation
Acid Phosphatase
Blotting, Western
Molecular Sequence Data
Biophysics
Bone Marrow Cells
Biology
Biochemistry
Cell Line
Mice
Multinucleate
Western blot
Calcitriol
Structural Biology
Internal medicine
Genetics
medicine
Animals
Northern blot
Amino Acid Sequence
Molecular Biology
Cells, Cultured
Osteoblasts
medicine.diagnostic_test
Osteoblastic cell
Osteoblast
Cell Differentiation
Cell Biology
Complement C3
Blotting, Northern
Molecular biology
medicine.anatomical_structure
Endocrinology
Liver
Cell culture
Electrophoresis, Polyacrylamide Gel
Bone marrow
Subjects
Details
- ISSN :
- 00145793
- Volume :
- 285
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- FEBS letters
- Accession number :
- edsair.doi.dedup.....8a4544cdf063dd417f06fecbaf073b9e