Back to Search
Start Over
Analytical techniques for characterization of biological molecules – proteins and aptamers/oligonucleotides
- Source :
- Bioanalysis. 11:103-117
- Publication Year :
- 2019
- Publisher :
- Future Science Ltd, 2019.
-
Abstract
- With the advent of the high-throughput technologies and exciting times for biology, the discipline of analytical methodology is experiencing a surge in the growth and the scope. Over the years, multitude of analytical techniques have evolved from a work-intensive, low sensitivity and high volume of reagent and sample consumption endeavor to automated, better selectivity, lower limit of quantification and cost-effective techniques for biological research. In this review, we give an overview of the currently available wide range of cell-based and noncell based and structural based analytical techniques, their principle and biological applications. The analytical techniques discussed in this paper includes surface plasmon resonance, electrophoresis, enzyme linked immunosorbent assay, Western blotting, flow cytometry, fluorescence activated cell sorting, mass spectrometry, nuclear magnetic resonance and x-ray crystallography.
- Subjects :
- Aptamer
Clinical Biochemistry
Oligonucleotides
Nanotechnology
Mass spectrometry
Sensitivity and Specificity
030226 pharmacology & pharmacy
01 natural sciences
Chemistry Techniques, Analytical
Analytical Chemistry
03 medical and health sciences
Fluorescence-Activated Cell Sorting
0302 clinical medicine
Animals
Humans
General Pharmacology, Toxicology and Pharmaceutics
Surface plasmon resonance
chemistry.chemical_classification
Oligonucleotide
Biomolecule
010401 analytical chemistry
Proteins
General Medicine
Aptamers, Nucleotide
0104 chemical sciences
Characterization (materials science)
Medical Laboratory Technology
Electrophoresis
chemistry
Subjects
Details
- ISSN :
- 17576199 and 17576180
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Bioanalysis
- Accession number :
- edsair.doi.dedup.....8a3c4661d7c72af8a6051c7037304050