Back to Search
Start Over
Embryonic MTHFR contributes to blastocyst development
- Source :
- J Assist Reprod Genet
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- PURPOSE: Reduction in methylenetetrahydrofolate reductase (MTHFR) activity due to genetic variations in the MTHFR gene has been controversially implicated in subfertility in human in vitro fertilization. However, there is no direct gene-knockdown study of embryonic MTHFR to assess its involvement in mammalian preimplantation development. The purpose of this study is to investigate expression profiles and functional roles of MTHFR in bovine preimplantation development. METHODS: Reverse transcription-quantitative PCR (RT-qPCR) and analysis of publicly available RNA-seq data were performed to reveal expression levels of MTHFR during bovine preimplantation development. We knocked down MTHFR by siRNA-mediated RNA interference from the 8- to 16-cell stage and assessed the effects on preimplantation development. RESULTS: The RT-qPCR analysis showed relatively high MTHFR expression at the GV oocyte stage, which was decreased toward the 8- to 16-cell stage and then slightly restored at the blastocyst stage. Public data–based analysis also showed the similar pattern of expression with substantial embryonic expression at the blastocyst stage. MTHFR knockdown reduced the blastocyst rate (P
- Subjects :
- 0301 basic medicine
medicine.medical_treatment
Embryonic Development
Andrology
03 medical and health sciences
0302 clinical medicine
Genetics
medicine
Animals
Humans
Inner cell mass
Blastocyst
RNA, Small Interfering
Methylenetetrahydrofolate Reductase (NADPH2)
Genetics (clinical)
Gene knockdown
030219 obstetrics & reproductive medicine
In vitro fertilisation
biology
Gene Expression Regulation, Developmental
Obstetrics and Gynecology
Embryo
General Medicine
Embryonic stem cell
digestive system diseases
Embryo Biology
Fertility
030104 developmental biology
medicine.anatomical_structure
Reproductive Medicine
In utero
Methylenetetrahydrofolate reductase
Oocytes
biology.protein
Cattle
Female
Developmental Biology
Subjects
Details
- ISSN :
- 15737330 and 10580468
- Volume :
- 37
- Database :
- OpenAIRE
- Journal :
- Journal of Assisted Reproduction and Genetics
- Accession number :
- edsair.doi.dedup.....8a1001b1ac64e6baf3f8611f8e073393