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Solid–liquid Interface Screening SystemーApplication to the Screening of Antibiotic and Cytotoxic Substance-producing Fungi
- Source :
- Biocontrol Science. 24:47-56
- Publication Year :
- 2019
- Publisher :
- The Society for Antibacterial and Antifungal Agents, Japan, 2019.
-
Abstract
- A useful tool for the screening of fungi producing biologically active secondary metabolites such as antibiotics and cytotoxic substances has been developed. An agar plate-organic solvent interface cultivation (A/S-IFC) system, which comprised a hydrophobic organic solvent (upper phase) , a fungal mat (middle phase) and an agar plate (lower phase) , was constructed. The metabolite profiles were compared among the A/S-IFC, a traditional submerged cultivation (SmC) and an extractive liquid surface immobilization (Ext-LSI) system consisted of a hydrophobic solvent (upper phase) , a fungal cells-ballooned microspheres (middle phase) and a liquid medium (lower phase) , with high-performance liquid chromatography-photodiode array detector (HPLC-PDA) . In the A/S-IFC, many hydrophobic metabolites vastly different from those in the SmC were accumulated in the organic phase as with the Ext-LSI. For example, a valuable azaphilone, sclerotiorin, was remarkably produced into the organic phase in the A/S-IFC. The A/S-IFC was applied to the screening of antibiotic-producing fungi. As a result of paper disk method, it was found that 321 isolated among 811 strains produced antifungal metabolites (hit rate, 39.6%) . Furthermore, 8, 23, and 30 strains also produced cytotoxic metabolites against SKOV-3 (human ovary adenocarcinoma) , MESO-1 (human malignant pleural mesothelioma) , and Jurkat cells (immortalized human T lymphocyte) .
- Subjects :
- 0106 biological sciences
Antifungal Agents
food.ingredient
Cell Survival
Metabolite
Antineoplastic Agents
01 natural sciences
Applied Microbiology and Biotechnology
Jurkat cells
Agar plate
03 medical and health sciences
chemistry.chemical_compound
food
Cell Line, Tumor
010608 biotechnology
Phase (matter)
Drug Discovery
Humans
Cytotoxic T cell
Agar
Benzopyrans
Chromatography, High Pressure Liquid
0303 health sciences
Chromatography
030306 microbiology
Fungi
Public Health, Environmental and Occupational Health
Biological activity
Pigments, Biological
Solvent
chemistry
Fermentation
Saccharomycetales
Solvents
Drug Screening Assays, Antitumor
Hydrophobic and Hydrophilic Interactions
Subjects
Details
- ISSN :
- 18840205 and 13424815
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- Biocontrol Science
- Accession number :
- edsair.doi.dedup.....8a0e76b3dc0470d44c76e89bf824550c