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Imaging the fetal nonhuman primate brain with SV2A positron emission tomography (PET)
- Source :
- European journal of nuclear medicine and molecular imaging, vol 49, iss 11, Eur J Nucl Med Mol Imaging
- Publication Year :
- 2022
- Publisher :
- eScholarship, University of California, 2022.
-
Abstract
- PURPOSE: Exploring synaptic density changes during brain growth is crucial to understanding brain development. Previous studies in nonhuman primates report a rapid increase in synapse number between the late gestational period and the early neonatal period, such that synaptic density approaches adult levels by birth. Prenatal synaptic development may have an enduring impact on postnatal brain development, but precisely how synaptic density changes in utero are unknown because current methods to quantify synaptic density are invasive and require post-mortem brain tissue. METHODS: We used synaptic vesicle glycoprotein 2A (SV2A) positron emission tomography (PET) radioligands [(11)C]UCB-J and [(18)F]Syn-VesT-1 to conduct the first assessment of synaptic density in the developing fetal brain in gravid rhesus monkeys. Eight pregnant monkeys were scanned twice during the third trimester at two imaging sites. Fetal post-mortem samples were collected near term in a subset of subjects to quantify SV2A density by Western blot. RESULTS: Image-derived fetal brain SV2A measures increased during the third trimester. SV2A concentrations were greater in subcortical regions than in cortical regions at both gestational ages. Near term, SV2A density was higher in primary motor and visual areas than respective associative regions. Post-mortem quantification of SV2A density was significantly correlated with regional SV2A PET measures. CONCLUSION: While further study is needed to determine the exact relationship of SV2A and synaptic density, the imaging paradigm developed in the current study allows for the effective in vivo study of SV2A development in the fetal brain.
- Subjects :
- Pediatric
Positron emission tomography
Membrane Glycoproteins
SV2A
Clinical Sciences
In utero
Neurosciences
Brain
Nerve Tissue Proteins
General Medicine
Reproductive health and childbirth
Perinatal Period - Conditions Originating in Perinatal Period
Macaca mulatta
Article
Brain Disorders
Other Physical Sciences
Nuclear Medicine & Medical Imaging
Nonhuman primate
Positron-Emission Tomography
Neurological
Animals
Biomedical Imaging
Radiology, Nuclear Medicine and imaging
Synaptogenesis
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- European journal of nuclear medicine and molecular imaging, vol 49, iss 11, Eur J Nucl Med Mol Imaging
- Accession number :
- edsair.doi.dedup.....8a04724910687e2903389725172c6605