Modified Screen-Printed Carbon Electrodes with Tyrosinase for Determination of Phenolic Compounds in Smoked Food

A screen-printed carbon electrode modified with tyrosinase (SPCE-Tyr/Paa/Glut) has been developed for the determination of phenol concentration in real samples. The resulting SPCE-Tyr/Paa/Glut was prepared in a one-step procedure, and was then optimized as an amperometric biosensor operating at 0 mV versus Ag/AgCl for phenol determination in flow injection mode. Phenol detection was realized by electrochemical reduction of quinone produced by tyrosinase activity. The possibility of using the developed biosensor to determine phenol concentrations in various smoked products (bacon, ham, chicken and salmon) was also evaluated. Gas chromatography (GC) method was used for result validation obtained in flow injection mode using amperometric biosensor. The result showed good correlation with those obtained by flowinjection analysis (FIA).