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3D spectral imaging with synchrotron Fourier transform infrared spectro-microtomography
- Source :
- Nature Methods. 10:861-864
- Publication Year :
- 2013
- Publisher :
- Springer Science and Business Media LLC, 2013.
-
Abstract
- We report Fourier transform infrared spectro-microtomography, a nondestructive three-dimensional imaging approach that reveals the distribution of distinctive chemical compositions throughout an intact biological or materials sample. The method combines mid-infrared absorption contrast with computed tomographic data acquisition and reconstruction to enhance chemical and morphological localization by determining a complete infrared spectrum for every voxel (millions of spectra determined per sample).
- Subjects :
- Chemical imaging
medicine.medical_specialty
Materials science
Infrared spectroscopy
Biochemistry
Fourier transform spectroscopy
Physics::Geophysics
Mice
symbols.namesake
Imaging, Three-Dimensional
Optics
Spectroscopy, Fourier Transform Infrared
Microscopy
Image Processing, Computer-Assisted
medicine
Animals
Humans
Fourier transform infrared spectroscopy
Molecular Biology
Embryonic Stem Cells
business.industry
X-Ray Microtomography
Cell Biology
Wood
Spectral imaging
Populus
Fourier transform
symbols
business
Chemical fingerprinting
Synchrotrons
Hair
Biotechnology
Subjects
Details
- ISSN :
- 15487105 and 15487091
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Nature Methods
- Accession number :
- edsair.doi.dedup.....89efde0b7268ab2ef73343c72007a800
- Full Text :
- https://doi.org/10.1038/nmeth.2596