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3D spectral imaging with synchrotron Fourier transform infrared spectro-microtomography

Authors :
Carol J. Hirschmugl
Jonathan M. Castro
Michael J. Nasse
Charlotte Dabat-Blondeau
Michael C. Martin
Hans A. Bechtel
David Buschke
Barbara L. Illman
Brenda M. Ogle
Miriam Unger
Julia Sedlmair
Marco Keiluweit
Dilworth Y. Parkinson
Source :
Nature Methods. 10:861-864
Publication Year :
2013
Publisher :
Springer Science and Business Media LLC, 2013.

Abstract

We report Fourier transform infrared spectro-microtomography, a nondestructive three-dimensional imaging approach that reveals the distribution of distinctive chemical compositions throughout an intact biological or materials sample. The method combines mid-infrared absorption contrast with computed tomographic data acquisition and reconstruction to enhance chemical and morphological localization by determining a complete infrared spectrum for every voxel (millions of spectra determined per sample).

Details

ISSN :
15487105 and 15487091
Volume :
10
Database :
OpenAIRE
Journal :
Nature Methods
Accession number :
edsair.doi.dedup.....89efde0b7268ab2ef73343c72007a800
Full Text :
https://doi.org/10.1038/nmeth.2596