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Abl-SH3 binding protein 2, 3BP2, interacts with CIN85 and HIP-55
- Source :
- FEBS Letters. (5):967-974
- Publisher :
- Federation of European Biochemical Societies. Published by Elsevier B.V.
-
Abstract
- The adapter 3BP2 is involved in leukocyte signaling downstream Src/Syk-kinases coupled immunoreceptors. Here, we show that 3BP2 directly interacts with the endocytic scaffold protein CIN85 and the actin-binding protein HIP-55. 3BP2 co-localized with CIN85 and HIP-55 in T cell rafts and at the T cell/APC synapse, an active zone of receptors and proteins recycling. A binding region of CIN85 SH3 domains on 3BP2 was mapped to a PVPTPR motif in the first proline-rich region of 3BP2, whereas the C-terminal SH3 domain of HIP-55 bound a more distal proline-rich domain of 3BP2. Together, our data suggest an unexpected role of 3BP2 in endocytic and cytoskeletal regulation through its interaction with CIN85 and HIP-55.
- Subjects :
- Scaffold protein
Proline
T-Lymphocytes
Amino Acid Motifs
Endocytic cycle
Biophysics
In Vitro Techniques
Biology
Models, Biological
Biochemistry
SH3 domain
Cell Line
src Homology Domains
Jurkat Cells
Structural Biology
Two-Hybrid System Techniques
Genetics
Humans
Amino Acid Sequence
HIP-55
Antigen-presenting cell
Cytoskeleton
Molecular Biology
Adaptor Proteins, Signal Transducing
Binding Sites
Microfilament Proteins
T-cell receptor
breakpoint cluster region
Cell Biology
Molecular biology
Endocytosis
Recombinant Proteins
Protein Structure, Tertiary
Cell biology
3BP2
Proline-rich domain
CIN85
Src homology 3 domain
Protein Binding
Proto-oncogene tyrosine-protein kinase Src
Subjects
Details
- Language :
- English
- ISSN :
- 00145793
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- FEBS Letters
- Accession number :
- edsair.doi.dedup.....89dfe520c791041c91246f0c57953264
- Full Text :
- https://doi.org/10.1016/j.febslet.2007.01.084