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Clinical, Ultrasonographic, Bacteriological, Cytological and Histopathological Findings of Uterine Involution in Ewes with Uterine Infection
- Source :
- Pathogens, Volume 9, Issue 1, Pathogens, Vol 9, Iss 1, p 54 (2020)
- Publication Year :
- 2020
- Publisher :
- MDPI, 2020.
-
Abstract
- The objectives of the study were (a) to study the characteristics of uterine involution in ewes that had developed subclinical uterine infection in the immediately post-partum period and (b) to evaluate effects of the infection in the subsequent reproductive performance of ewes. Uterine infection was induced in ewes (I, n = 10) by intrauterine inoculation of Escherichia coli<br />uninoculated controls were included (C, n = 12). Animals were examined at regular intervals before and post-inoculation. Clinical and ultrasonographic examinations were performed. Vaginal swab samples and biopsy uterine tissue samples were collected for bacteriological, cytological and histological examination. Finally, ewes were put to rams and reproductive performance was monitored. After challenge, it was ultrasonographically found that caruncular dimensions, myometrial thickness and diameter of uterine lumen were greater in I ewes. In these ewes, particular reduction of dimensions occurred during the second week post-partum, whilst in C ewes during the first week. The uterine artery diameter and the blood flow into the uterus were also greater in I than in C ewes. E. coli infection was more frequent and of longer duration in I than in C ewes: in 68.1% and 50.0% of ewes and 19.5 and 14 days, respectively. There was lower proportion of neutrophils and higher of lymphocytes in group I than in C. In inoculated ewes, there was histological evidence of uterine epithelial destruction, increased cellular infiltration, hyperaemia and extracasation, which persisted up to 42 days post-partum. During the subsequent reproductive season, all ewes in group I lambed normally and produced healthy and viable lambs. No significant difference in reproductive performance parameters were seen in I comparison to C ewes. It is concluded that the innate immunity of the uterus sufficed to counteract the bacterial infection, although the process of involution took longer than in healthy animals<br />moreover, the ultrasonographic examination is a useful means for assessment of the genital tract of ewes post-partum<br />finally, no adverse effects were noted in the subsequent reproductive performance of ewes.
- Subjects :
- Microbiology (medical)
endometritis
sheep
040301 veterinary sciences
Uterus
lcsh:Medicine
Physiology
Article
0403 veterinary science
medicine.artery
Biopsy
medicine
Immunology and Allergy
Involution (medicine)
Metritis
Uterine artery
Molecular Biology
Subclinical infection
General Immunology and Microbiology
medicine.diagnostic_test
business.industry
metritis
Uterine Involution
lcsh:R
0402 animal and dairy science
Doppler
04 agricultural and veterinary sciences
pregnancy toxaemia
medicine.disease
040201 dairy & animal science
reproductive performance
Infectious Diseases
medicine.anatomical_structure
Endometritis
business
Subjects
Details
- Language :
- English
- ISSN :
- 20760817
- Volume :
- 9
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Pathogens
- Accession number :
- edsair.doi.dedup.....89ab270fb2eb2c0613dd71c36d38ac54