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Hsp65-producing Lactococcus lactis prevents experimental autoimmune encephalomyelitis in mice by inducing CD4+LAP+ regulatory T cells
- Source :
- Journal of Autoimmunity. 40:45-57
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- Heat shock proteins (Hsps) participate in the cellular response to stress and they are hiperexpressed in inflammatory conditions. They are also known to play a major role in immune modulation, controlling, for instance, autoimmune responses. In this study, we showed that oral administration of a recombinant Lactococcus lactis strain that produces and releases LPS-free Hsp65 prevented the development of experimental autoimmune encephalomyelitis (EAE) in C57BL/6 mice. This was confirmed by the reduced inflammatory cell infiltrate and absence of injury signs in the spinal cord. The effect was associated with reduced IL-17 and increased IL-10 production in mesenteric lymph node and spleen cell cultures. Hsp65-producing-L. lactis-fed mice had a remarkable increase in the number of natural and inducible CD4+Foxp3+ regulatory T (Treg) cells and CD4+LAP+ (Latency-associated peptide) Tregs - which express the membrane-bound TGF-β - in spleen, inguinal and mesenteric lymph nodes as well as in spinal cord. Moreover, many Tregs co-expressed Foxp3 and LAP. In vivo depletion of LAP+ cells abrogated the effect of Hsp65-producing L. lactis in EAE prevention and worsened disease in medium-fed mice. Thus, Hsp65-L.lactis seems to boost this critical regulatory circuit involved in controlling EAE development in mice.
- Subjects :
- Male
Encephalomyelitis, Autoimmune, Experimental
Encephalomyelitis
Immunology
Autoimmunity
Spleen
T-Lymphocytes, Regulatory
Article
Mice
Bacterial Proteins
Transforming Growth Factor beta
Heat shock protein
medicine
Animals
Immunology and Allergy
Mesenteric lymph nodes
biology
Lactococcus lactis
Experimental autoimmune encephalomyelitis
FOXP3
Forkhead Transcription Factors
Chaperonin 60
Transforming growth factor beta
biology.organism_classification
medicine.disease
Mice, Inbred C57BL
Mycobacterium leprae
medicine.anatomical_structure
Spinal Cord
CD4 Antigens
biology.protein
Female
Lymph Nodes
Subjects
Details
- ISSN :
- 08968411
- Volume :
- 40
- Database :
- OpenAIRE
- Journal :
- Journal of Autoimmunity
- Accession number :
- edsair.doi.dedup.....89a6838fa600254473a35751ca2859db