Supplementary Figures 1-7 from Menin Epigenetically Represses Hedgehog Signaling in MEN1 Tumor Syndrome

PDF file - 2MB, Supplementary Fig. S1. Menin interacts directly with PRMT5.Supplementary Fig. S2. Men1 is completely excised in Men1l/l;Ubc9CreER mice after treatment with tamoxifen (4OHT). Supplementary Fig. S3. Gene Set Enrichment Analysis (GSEA) of control Men1l/l and Men1-excised islets. Supplementary Fig. S4. Menin recruits PRMT5 and its histone methylation mark, H4R3m2s, to the Gas1 promoter. Supplementary Fig. S5. Ectopic expression of PRMT5, and either wild type or mutant menin in HEK293 cells used for co-immunoprecipitation and histone methyltransferase (HMT) assays. Supplementary Fig. S6. Islet area and fasting insulin levels in Men1l/l; RipCre mice treated with either control solution or GDC 0449.Supplementary Fig. S7. Menin interacts with PRMT5, but not PRMT7. Menin was ectopically expressed in HEK293 cells, and menin immunoprecipitates were immunoblotted with either anti-PRMT5 or -PRMT7 antibodies.