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A Convergent Functional Genomics Analysis to Identify Biological Regulators Mediating Effects of Creatine Supplementation
- Source :
- Nutrients, Nutrients, Vol 13, Iss 2521, p 2521 (2021), Volume 13, Issue 8
- Publication Year :
- 2021
-
Abstract
- Creatine (Cr) and phosphocreatine (PCr) are physiologically essential molecules for life, given they serve as rapid and localized support of energy- and mechanical-dependent processes. This evolutionary advantage is based on the action of creatine kinase (CK) isozymes that connect places of ATP synthesis with sites of ATP consumption (the CK/PCr system). Supplementation with creatine monohydrate (CrM) can enhance this system, resulting in well-known ergogenic effects and potential health or therapeutic benefits. In spite of our vast knowledge about these molecules, no integrative analysis of molecular mechanisms under a systems biology approach has been performed to date<br />thus, we aimed to perform for the first time a convergent functional genomics analysis to identify biological regulators mediating the effects of Cr supplementation in health and disease. A total of 35 differentially expressed genes were analyzed. We identified top-ranked pathways and biological processes mediating the effects of Cr supplementation. The impact of CrM on miRNAs merits more research. We also cautiously suggest two dose–response functional pathways (kinase- and ubiquitin-driven) for the regulation of the Cr uptake. Our functional enrichment analysis, the knowledge-based pathway reconstruction, and the identification of hub nodes provide meaningful information for future studies. This work contributes to a better understanding of the well-reported benefits of Cr in sports and its potential in health and disease conditions, although further clinical research is needed to validate the proposed mechanisms.
- Subjects :
- 0301 basic medicine
Phosphocreatine
Systems biology
Computational biology
Creatine
Article
03 medical and health sciences
chemistry.chemical_compound
Mice
0302 clinical medicine
sodium-chloride-dependent neurotransmitter symporters
Neurotransmitter Transport Proteins
Animals
Humans
TX341-641
Nutrition and Dietetics
biology
Nutrition. Foods and food supply
Kinase
creatine kinase
Gene Expression Profiling
systems biology
Genomics
bioinformatics
Physical Functional Performance
MAP kinase signaling system
030104 developmental biology
chemistry
Dietary Supplements
biology.protein
Creatine kinase
Creatine Monohydrate
Signal transduction
Mitogen-Activated Protein Kinases
Energy Metabolism
Functional genomics
030217 neurology & neurosurgery
signal transduction
Food Science
Genome-Wide Association Study
Subjects
Details
- ISSN :
- 20726643
- Volume :
- 13
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Nutrients
- Accession number :
- edsair.doi.dedup.....899c85355fe1235fa26f2cdf97c0af5f