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A Convergent Functional Genomics Analysis to Identify Biological Regulators Mediating Effects of Creatine Supplementation

Authors :
Michael D. Roberts
Richard B. Kreider
Diego A. Bonilla
Diego A. Forero
Yurany Moreno
Jeffrey R. Stout
Chad M. Kerksick
Eric S. Rawson
Source :
Nutrients, Nutrients, Vol 13, Iss 2521, p 2521 (2021), Volume 13, Issue 8
Publication Year :
2021

Abstract

Creatine (Cr) and phosphocreatine (PCr) are physiologically essential molecules for life, given they serve as rapid and localized support of energy- and mechanical-dependent processes. This evolutionary advantage is based on the action of creatine kinase (CK) isozymes that connect places of ATP synthesis with sites of ATP consumption (the CK/PCr system). Supplementation with creatine monohydrate (CrM) can enhance this system, resulting in well-known ergogenic effects and potential health or therapeutic benefits. In spite of our vast knowledge about these molecules, no integrative analysis of molecular mechanisms under a systems biology approach has been performed to date<br />thus, we aimed to perform for the first time a convergent functional genomics analysis to identify biological regulators mediating the effects of Cr supplementation in health and disease. A total of 35 differentially expressed genes were analyzed. We identified top-ranked pathways and biological processes mediating the effects of Cr supplementation. The impact of CrM on miRNAs merits more research. We also cautiously suggest two dose–response functional pathways (kinase- and ubiquitin-driven) for the regulation of the Cr uptake. Our functional enrichment analysis, the knowledge-based pathway reconstruction, and the identification of hub nodes provide meaningful information for future studies. This work contributes to a better understanding of the well-reported benefits of Cr in sports and its potential in health and disease conditions, although further clinical research is needed to validate the proposed mechanisms.

Details

ISSN :
20726643
Volume :
13
Issue :
8
Database :
OpenAIRE
Journal :
Nutrients
Accession number :
edsair.doi.dedup.....899c85355fe1235fa26f2cdf97c0af5f