Production of 10-hydroxy-12,15(Z,Z)-octadecadienoic acid from α-linolenic acid by permeabilized Stenotrophomonas nitritireducens cells

To improve the production of 10-hydroxy-12,15(Z,Z)-octadecadienoic acid (HODA) from α-linolenic acid in Stenotrophomonas nitritireducens. Cells of the bacterium were permeabilized with 1.25% (v/v) methanol. The optimal conditions for HODA production by permeabilized cells were pH 7, 35 °C, 5% (v/v) DMSO, 50 g cells l−1, and 22.5 g α-linolenic acid l−1. Under these conditions, permeabilized cells produced 16.4 g HODA l−1 after 2 h, with a conversion yield of 73 % (w/w) and a volumetric productivity of 8.2 g l−1 h−1. These values were 153 and 230 % of the values for non-permeabilized cells This is the highest concentration and volumetric and specific productivities of HODA reported thus far.