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Intracellular parasitism by the human granulocytic ehrlichiosis bacterium through the P-selectin ligand, PSGL-1
- Source :
- Science (New York, N.Y.). 288(5471)
- Publication Year :
- 2000
-
Abstract
- Human granulocytic ehrlichiosis (HGE) is a febrile tick-borne illness caused by a recently discovered intracellular bacterium remarkable for its tropism for professionally phagocytic neutrophils. Monoclonal antibodies against the P-selectin binding domain of the leukocyte P-selectin glycoprotein ligand, PSGL-1, prevented HGE cell binding and infection, as did enzymatic digestion of PSGL-1. Furthermore, simultaneous neoexpression in nonsusceptible cells of complementary DNAs for both PSGL-1 and its modifying α-(1,3) fucosyltransferase, Fuc-TVII, allowed binding and infection by HGE. Thus, the HGE bacterium specifically bound to fucosylated leukocyte PSGL-1. Selectin mimicry is likely central to the organism's unique ability to target and infect neutrophils.
- Subjects :
- Ehrlichiosis
Fucosyltransferase
Glycosylation
P-selectin
medicine.drug_class
Neutrophils
Ehrlichia
Oligosaccharides
HL-60 Cells
Monoclonal antibody
Ligands
Transfection
Cell Line
medicine
Humans
Fluorescent Antibody Technique, Indirect
Sialyl Lewis X Antigen
Tropism
chemistry.chemical_classification
B-Lymphocytes
Multidisciplinary
Membrane Glycoproteins
integumentary system
biology
Molecular Mimicry
Antibodies, Monoclonal
Metalloendopeptidases
medicine.disease
Fucosyltransferases
Molecular biology
P-Selectin
chemistry
biology.protein
Glycoprotein
Selectin
Binding domain
Granulocytes
Subjects
Details
- ISSN :
- 00368075
- Volume :
- 288
- Issue :
- 5471
- Database :
- OpenAIRE
- Journal :
- Science (New York, N.Y.)
- Accession number :
- edsair.doi.dedup.....89971d964a13abd26ca0eaeffb79f30d