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CNS midline cells contribute to maintenance of the initial dorsoventral patterning of theDrosophila ventral neuroectoderm
- Source :
- Journal of Neurobiology. 62:397-405
- Publication Year :
- 2005
- Publisher :
- Wiley, 2005.
-
Abstract
- Dorsoventral patterning of the Drosophila ventral neuroectoderm is established by the expression of three evolutionarily conserved homeodomain genes: ventral nervous system defective (vnd), intermediate neuroblasts defective (ind), and muscle segment homeobox (msh) in the medial, intermediate, and lateral columns of the ventral neuroectoderm, respectively. It was not clear whether extrinsic factor(s) from the CNS midline cells influence the initial dorsoventral patterning by controlling the expression of the dorsoventral patterning genes. We show here that the CNS midline cells, specified by single-minded (sim), are essential for maintaining expression of the dorsoventral patterning genes. Ectopic expression of sim in the ventral neuroectoderm during the blastoderm stage repressed expression of the three homeodomain genes in the ventral neuroectoderm. This indicates that the identity of the CNS midline cells is established by a series of repressions of the three homeodomain genes in the ventral neuroectoderm. Ectopic expression of sim in the ventral neuroectoderm during initial neurogenesis induced ectopic ind expression in the medial column in addition to that in the intermediate column via EGFR signaling between the ventral neuroectoderm and midline cells. In contrast, it repressed the expression of vnd and msh in the medial and lateral columns, respectively. Our findings demonstrate that the CNS midline cells provide extrinsic positional information via EGFR signaling that maintains the initial subdivision of the ventral neuroectoderm into three dorsoventral columns during initial neurogenesis.
- Subjects :
- Central Nervous System
Nervous system
endocrine system
animal structures
Neuroectoderm
General Neuroscience
Neurogenesis
Gene Expression Regulation, Developmental
Biology
Cellular and Molecular Neuroscience
SOX1
medicine.anatomical_structure
Neuroblast
Ectoderm
embryonic structures
medicine
Animals
Drosophila Proteins
Homeobox
Drosophila
Ectopic expression
Neuroscience
Blastoderm
Subjects
Details
- ISSN :
- 10974695 and 00223034
- Volume :
- 62
- Database :
- OpenAIRE
- Journal :
- Journal of Neurobiology
- Accession number :
- edsair.doi.dedup.....899522c4316087a19256d0d4ee86ece4