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Localization of prolidase gene expression in scar tissue using in situ hybridization
- Source :
- Journal of Dermatological Science. 12:163-171
- Publication Year :
- 1996
- Publisher :
- Elsevier BV, 1996.
-
Abstract
- We investigated rolidase gene expression in human skin by means of Northern blot analysis and in situ hybridization. Northern blot analysis revealed that an mRNA species that was specific for prolidase was present in cultured human skin fibroblasts and keratinocytes. In situ hybridization using non-isotopic riboprobes labeled with digoxigenin and an isotopic riboprobe labeled with [35S]UTP localized prolidase gene expression to fibroblasts and endothelial cells of small vessels in scar tissue. Prolidase mRNA was also prominently expressed in keratinocytes near the basal layer overlying scar tissue. These findings indicate that prolidase may have an important role in wound healing.
- Subjects :
- Adult
Keratinocytes
Male
Dipeptidases
Adolescent
Gene Expression
Human skin
Dermatology
In situ hybridization
Biology
Biochemistry
Cicatrix
chemistry.chemical_compound
Gene expression
Humans
Digoxigenin
Tissue Distribution
RNA, Messenger
Northern blot
Child
Molecular Biology
In Situ Hybridization
Aged
Skin
Aged, 80 and over
Messenger RNA
Riboprobe
Fibroblasts
Middle Aged
Blotting, Northern
Molecular biology
chemistry
Female
Wound healing
Subjects
Details
- ISSN :
- 09231811
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Journal of Dermatological Science
- Accession number :
- edsair.doi.dedup.....897c210b2060cd82f7dd79e716f08954