Back to Search
Start Over
Preventive effects of anthraquinone food pigments on the DNA damage induced by carcinogens in Drosophila
- Source :
- Mutation research.
- Publication Year :
- 2001
-
Abstract
- We have previously demonstrated the inhibitory effect of chlorophyllin, a green food additive, on the genotoxicities of various carcinogens in Drosophila. Recently, we reported that purpurin, a component of a red food additive produced from madder root (Rubia tinctorium), inhibits the bacterial mutagenicity of heterocyclic amines. In the present study, we examined antigenotoxic activities of various pigments that are either constituents of food or food additives, using Drosophila in vivo DNA repair assay. Third instar larvae of Drosophila were fed a mutagen with or without pigment. The resulting adult flies were monitored for their male (repair deficient)/female (repair proficient) ratios, which reflect the DNA damage. We tested a total of 20 pigments, which are mainly of plant origins, including flavonoids, carotenoids, anthocyanins, anthraquinones and beta-diketone (curcumin)-derivatives, against the genotoxicities of eight carcinogens; IQ, MeIQx, AFB1, NDMA, 2-AAF, DMBA, 4NQO, and MNU. Four anthraquinone pigments (alizarin, purpurin, lac color, and cochineal extract) showed significant antigenotoxic activities. Alizarin and purpurin suppressed the DNA damage induced by IQ, MeIQx, AFB1, NDMA, 2-AAF, DMBA, and MNU. Lac color and cochineal extract showed inhibition against IQ, MeIQx, AFB1, 2-AAF and DMBA. In these inhibitions, suppression of metabolic enzymes may be involved. Since purpurin and alizarin suppressed the activity of MNU, a direct alkylating agent, there may also be a mechanism distinct from enzyme inhibitions in these anthraquinone-mediated suppressions of DNA damage.
- Subjects :
- Male
DNA damage
DNA repair
Health, Toxicology and Mutagenesis
DMBA
Mutagen
Anthraquinones
Biology
medicine.disease_cause
Alizarin
Carmine
Chemoprevention
chemistry.chemical_compound
Genetics
medicine
Animals
Molecular Biology
Carcinogen
Dose-Response Relationship, Drug
Mutagenicity Tests
Chlorophyllin
fungi
Food Coloring Agents
Antimutagenic Agents
Biochemistry
chemistry
Carcinogens
Biological Assay
Drosophila
Female
Genotoxicity
DNA Damage
Subjects
Details
- ISSN :
- 00275107
- Database :
- OpenAIRE
- Journal :
- Mutation research
- Accession number :
- edsair.doi.dedup.....897b59f668bd3e7f4271d439aa4b72b4