Back to Search
Start Over
A paper-based chemiluminescence immunoassay device for rapid and high-throughput detection of allergen-specific IgE
- Source :
- The Analyst. 144(8)
- Publication Year :
- 2019
-
Abstract
- The fast and precise detection of potential allergen-specific immunoglobulin E (sIgE) is imperative for the diagnosis and appropriate treatment of allergic diseases. In this study, we have successfully fabricated a novel paper-based immunoassay device for the detection of sIgE in allergic diseases. We used Can f 1, one of the main dog allergens, as a model allergen to detect sIgE in human sera. To achieve excellent performance, the experimental parameters were optimized. Further, we extended this device for potential applications in the clinical diagnosis of allergic diseases: worthwhile clinical performance in the detection of allergens was achieved as compared to that achieved by commercial enzyme-linked immunosorbent assay (ELISA) kit. Therefore, it was proven that this strategy has the advantages of high-throughput, rapid, sensitive, and highly accurate detection of trace amounts of sIgEs. Furthermore, by simply changing the antigen and antibody, this device could be used for the high-throughput detection of other allergens, so as to achieve multiallergen detection and appropriate desensitization therapy, thereby making it promising in the determination of allergic diseases in clinics.
- Subjects :
- Paper
Luminescence
Chemiluminescence immunoassay
Enzyme-Linked Immunosorbent Assay
02 engineering and technology
Immunoglobulin E
medicine.disease_cause
01 natural sciences
Biochemistry
Armoracia
Analytical Chemistry
Allergen
Electrochemistry
medicine
Escherichia coli
Hypersensitivity
Environmental Chemistry
Animals
Humans
Allergen specific IgE
Spectroscopy
Horseradish Peroxidase
biology
medicine.diagnostic_test
business.industry
fungi
010401 analytical chemistry
Temperature
Reproducibility of Results
Serum Albumin, Bovine
Paper based
Allergens
Hydrogen-Ion Concentration
021001 nanoscience & nanotechnology
0104 chemical sciences
Clinical diagnosis
Immunoassay
Immunology
Luminescent Measurements
biology.protein
Cattle
Luminol
0210 nano-technology
business
Oxidation-Reduction
Desensitization therapy
Subjects
Details
- ISSN :
- 13645528
- Volume :
- 144
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- The Analyst
- Accession number :
- edsair.doi.dedup.....897a405930a6a0d45be1aea7ccd12c9d