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Effect of Th1/Th2 Cytokine Pretreatment on RSV-Induced Gene Expression in Airway Epithelial Cells
- Source :
- International Archives of Allergy and Immunology. 154:185-194
- Publication Year :
- 2010
- Publisher :
- S. Karger AG, 2010.
-
Abstract
- Background: Respiratory syncytial virus (RSV) infection in infants with Th2 predisposition is thought to increase the risk of allergic sensitization, recurrent wheezing, and bronchial asthma during childhood. We attempted to clarify the molecular mechanisms by which Th1/Th2 predisposition in the host alters RSV infection and facilitates airway inflammation. Methods: A549 human airway epithelial cells were inoculated with live or UV-treated RSV after pretreatment with either a combination of tumor necrosis factor (TNF)-α and interferon-γ (Th1-primed) or a combination of TNF-α and interleukin-4 (Th2-primed) for 48 h. The gene and protein expression profiles of RSV-infected A549 cells were examined. Results: GeneChip analysis indicated that, at 96 h after inoculation with RSV, the expression of 62 genes was specifically enhanced (more than 2-fold by normalized data) in Th2-primed cells compared to that in unprimed or Th1-primed cells. An increase in mRNA and protein levels of monocyte chemoattractant protein (MCP)-1/CCL2 among those 62 genes was confirmed by real-time PCR and cytometric bead assay, respectively. RSV replication was markedly diminished in Th1-primed airway epithelial cells but not in Th2-primed cells, which was presumably caused at least in part by the early induction of antiviral genes. Conclusions: These results suggest that Th1/Th2 predisposition in the host prior to RSV infection critically regulates inflammatory reactions in the airways through alteration of gene expression, and that MCP-1/CCL2 plays an important role in the pathogenesis of severe RSV infection and the subsequent development of asthma in Th2-predisposed hosts.
- Subjects :
- Time Factors
medicine.medical_treatment
Immunology
Biology
CCL2
Virus Replication
Cell Line
Pathogenesis
Interferon-gamma
Th2 Cells
Gene expression
medicine
Humans
Immunology and Allergy
Lung
Chemokine CCL2
Oligonucleotide Array Sequence Analysis
A549 cell
Regulation of gene expression
Reverse Transcriptase Polymerase Chain Reaction
Tumor Necrosis Factor-alpha
Gene Expression Profiling
Epithelial Cells
General Medicine
Th1 Cells
respiratory system
Cytokine
Gene Expression Regulation
Cell culture
Respiratory Syncytial Virus, Human
Cytokines
Tumor necrosis factor alpha
Subjects
Details
- ISSN :
- 14230097 and 10182438
- Volume :
- 154
- Database :
- OpenAIRE
- Journal :
- International Archives of Allergy and Immunology
- Accession number :
- edsair.doi.dedup.....895dbc95f982b44b097a88c2c3ede086