Modulation of Differentiation of Embryonic Stem Cells by Polypyrrole: The Impact on Neurogenesis

The active role of biomaterials in the regeneration of tissues and their ability to modulate the behavior of stem cells in terms of their differentiation is highly advantageous. Here, polypyrrole, as a representantive of electro-conducting materials, is found to modulate the behavior of embryonic stem cells. Concretely, the aqueous extracts of polypyrrole induce neurogenesis within embryonic bodies formed from embryonic stem cells. This finding ledto an effort to determine the physiological cascade which is responsible for this effect. The polypyrrole modulates signaling pathways of Akt and ERK kinase through their phosphorylation. These effects are related to the presence of low-molecularweight compounds present in aqueous polypyrrole extracts, determined by mass spectroscopy. The results show that consequences related to the modulation of stem cell differentiation must also be taken into account when polypyrrole is considered as a biomaterial. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Czech Science FoundationGrant Agency of the Czech Republic [19-16861S, 17-05466S]; internal grant agency of Tomas Bata University in Zlin [IGA/CPS/2021/001]; Slovenian Research AgencySlovenian Research Agency - Slovenia [P2-0082]; Ministry of Education, Youth and Sports of the Czech Republic-DKRVO [RP/CPS/2020/001]
RP/CPS/2020/001; Ministerstvo Školství, Mládeže a Tělovýchovy, MŠMT; Grantová Agentura České Republiky, GA ČR: 17-05466S, 19-16861S; Javna Agencija za Raziskovalno Dejavnost RS, ARRS: P2-0082; Univerzita Tomáše Bati ve Zlíně: IGA/CPS/2021/001