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Impaired alternative macrophage differentiation of peripheral blood mononuclear cells from obese subjects.: Macrophage alternative differentiation and obesity
- Source :
- Diabetes and Vascular Disease Research, Diabetes and Vascular Disease Research, 2012, 9 (3), pp.189-95. ⟨10.1177/1479164111430242⟩, Diabetes and Vascular Disease Research; Vol 9, Diabetes and Vascular Disease Research, SAGE Publications, 2012, 9 (3), pp.189-95. ⟨10.1177/1479164111430242⟩
- Publication Year :
- 2011
- Publisher :
- Sage Publications, 2011.
-
Abstract
- International audience; Visceral obesity is a chronic, low-grade inflammatory disease that predisposes people to the metabolic syndrome, type 2 diabetes and its cardiovascular complications. Adipose tissue is not a passive storehouse for fat, but an endocrine organ synthesizing and releasing a variety of bioactive molecules, some of which are produced by infiltrated immune-inflammatory cells including macrophages. Two different subpopulations of macrophages have been identified in adipose tissue: pro-inflammatory 'classical' M1 and anti-inflammatory 'alternative' M2 macrophages, and their ratio is suggested to influence the metabolic complications of obesity. These macrophages derive primarily from peripheral blood mononuclear cells (PBMCs). We hypothesised that obesity and the metabolic syndrome modulate PBMC functions. Therefore, alteration of the monocyte response, and more specifically their ability to differentiate toward alternative anti-inflammatory macrophages, was assessed in PBMCs isolated from lean and obese subjects with or without alterations in glucose homeostasis. Our results indicate that PBMCs from obese subjects have an altered expression of M2 markers and that their monocytes are less susceptible to differentiate toward an alternative phenotype. Thus PBMCs in obesity are programmed, which may contribute to the inflammatory dysregulation and increased susceptibility to inflammatory diseases in these patients.
- Subjects :
- Adult
medicine.medical_specialty
Endocrinology, Diabetes and Metabolism
Adipose tissue macrophages
Adipose tissue
Inflammation
030204 cardiovascular system & hematology
Peripheral blood mononuclear cell
Article
03 medical and health sciences
0302 clinical medicine
Internal medicine
Diabetes mellitus
Internal Medicine
Medicine
Glucose homeostasis
Humans
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
Obesity
030304 developmental biology
0303 health sciences
polarization
business.industry
Monocyte
Gene Expression Profiling
Macrophages
Cell Differentiation
Middle Aged
medicine.disease
Endocrinology
medicine.anatomical_structure
Phenotype
Adipose Tissue
Diabetes Mellitus, Type 2
Immunology
gene expression
Leukocytes, Mononuclear
Female
Metabolic syndrome
medicine.symptom
Inflammation Mediators
Cardiology and Cardiovascular Medicine
business
Subjects
Details
- Language :
- English
- ISSN :
- 14791641 and 17528984
- Database :
- OpenAIRE
- Journal :
- Diabetes and Vascular Disease Research, Diabetes and Vascular Disease Research, 2012, 9 (3), pp.189-95. ⟨10.1177/1479164111430242⟩, Diabetes and Vascular Disease Research; Vol 9, Diabetes and Vascular Disease Research, SAGE Publications, 2012, 9 (3), pp.189-95. ⟨10.1177/1479164111430242⟩
- Accession number :
- edsair.doi.dedup.....895534bdc612bf30070854f8f8479770